PEPLASER
Combinatorial synthesis of peptide arrays with a laser printer
| Coordinatore | Karlsruher Institut fuer Technologie
Organization address
address: Kaiserstrasse 12 contact info |
| Nazionalità Coordinatore | Germany [DE] |
| Totale costo | 4˙028˙614 € |
| EC contributo | 2˙988˙450 € |
| Programma | FP7-HEALTH
Specific Programme "Cooperation": Health |
| Code Call | FP7-HEALTH-2007-B |
| Funding Scheme | CP-TP |
| Anno di inizio | 2009 |
| Periodo (anno-mese-giorno) | 2009-01-01 - 2011-12-31 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
Karlsruher Institut fuer Technologie
Organization address
address: Kaiserstrasse 12 contact info |
DE (Karlsruhe) | coordinator | 313˙766.70 |
| 2 |
AKAtech Produktions- und Handels GmbH
Organization address
city: Frankenmarkt contact info |
AT (Frankenmarkt) | participant | 600˙000.00 |
| 3 |
KMS Automation GmbH
Organization address
address: Im Webertal 20 contact info |
DE (Schramberg-Waldmoesssingen) | participant | 550˙610.00 |
| 4 |
FRAUNHOFER-GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V
Organization address
address: Hansastrasse 27C contact info |
DE (MUENCHEN) | participant | 300˙000.00 |
| 5 |
UPPER AUSTRIAN RESEARCH GMBH
Organization address
address: Hafenstrasse 47-51 contact info |
AT (Linz) | participant | 298˙800.00 |
| 6 |
BIOINFOBANK INSTITUTE
Organization address
address: Limanowskiego 24/a 16 contact info |
PL (POZNAN) | participant | 180˙000.00 |
| 7 |
MICROSYSTEMS LTD
Organization address
address: kv. CHAIKA 15-D-21 contact info |
BG (Varna) | participant | 150˙000.00 |
| 8 |
TECHNICAL UNIVERSITY OF VARNA
Organization address
address: Studentska 1 contact info |
BG (VARNA) | participant | 150˙000.00 |
| 9 |
DEUTSCHES KREBSFORSCHUNGSZENTRUM
Organization address
address: Im Neuenheimer Feld 280 contact info |
DE (HEIDELBERG) | participant | 145˙233.31 |
| 10 |
AIMS Scientific Products GmbH
Organization address
city: Braunschweig contact info |
DE (Braunschweig) | participant | 100˙080.00 |
| 11 |
PEPperPRINT GmbH
Organization address
address: Schlossweg 9 contact info |
DE (Wiesloch) | participant | 100˙000.00 |
| 12 |
Selvita spolka z ograniczona odpowiedzialnoscia
Organization address
address: ul. Gersona 2 contact info |
PL (KRAKOW) | participant | 99˙960.00 |
Mappa
Word cloud
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Obiettivo del progetto (Objective)
'Our particle-based method allows us to synthesise high complexity peptide arrays by combinatorial synthesis and for an unrivalled prize. We plan to further develop this new technology up to the level of robust prototype machines, and mate it to bioinformatics and readout tools. Together, our procedure(s) should boost the field of proteomics in a similar way as the lithographic technologies did with the field of genomics. Central to our novel method are the activated chemical building blocks that are “frozen” within solid amino acid particles. Thereby, we can use a colour laser printer to send them to defined addresses on a 2D support, where the particles are simply melted to induce a spatially defined coupling reaction of now freed amino acid derivatives. By repeated printing and melting cycles this simple trick yields high complexity peptide arrays. Based on existing pre-prototypes, we will develop a user-friendly peptide laser printer that spatially defined addresses our 20 different amino acid toners in high resolution to a support (WP1), and a scanner that especially fast and sensitive reads out the large formats delivered by the peptide laser printer (WP2). The increased production of amino acid toners and array supports are other bottlenecks in the output of peptide arrays that are tackled in WP3. This should allow us to increase the output of individual peptide spots from currently 0,5 Million to >10 Million peptides per month. Finally, to foster a market for high complexity peptide arrays, we will work out paradigmatic application examples in WP4. These aim to directly screen for antibiotic or apoptosis inducing D-peptides, and for the comprehensive readout of the different antibodies that patrol the serum of autoimmune patients. Based on user-friendly prototype machines, on first paradigmatic application examples for high complexity peptide arrays, and shielded by a strong patent, the participating SMEs will commercialise this new technology.'
Introduzione (Teaser)
If the genome seems unfathomable, imagine trying to identify minute pieces of all the products it codes for. Scientists have made this possible with a device for rapid and cost-effective identification of small parts of selected proteins.