FORCEFULACTIN

Coordinated regulation of actin dynamics in cell motility and morphogenesis : from molecules to reconstituted biomimetic assays

Coordinatore	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	France [FR]
Totale costo	2˙434˙195 €
EC contributo	2˙434˙195 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2009-AdG
Funding Scheme	ERC-AG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-05-01   -   2015-10-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	TECHNISCHE UNIVERSITAT BRAUNSCHWEIG  Organization address address: POCKELSSTRASSE 14 city: BRAUNSCHWEIG postcode: 38106 contact info Titolo: Prof. Nome: Klemens Cognome: Rottner Email: send email Telefono: +49 531 391 3255	DE (BRAUNSCHWEIG)	beneficiary	127˙886.86
2	RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAT BONN  Organization address address: REGINA PACIS WEG 3 city: BONN postcode: 53113 contact info Nome: Daniela Cognome: Hasenpusch Email: send email Telefono: +49 228737274 Fax: +49228 736479	DE (BONN)	beneficiary	112˙113.14
3	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Dr. Nome: Marie-France Cognome: Carlier épouse Pantaloni Email: send email Telefono: +33 1 69823465 Fax: +33 1 69 82 31 29	FR (PARIS)	hostInstitution	2˙194˙195.00
4	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE  Organization address address: Rue Michel -Ange 3 city: PARIS postcode: 75794 contact info Titolo: Ms. Nome: Véronique Cognome: Debisschop Email: send email Telefono: +33 1 69 82 32 64 Fax: +33 1 69 82 33 00	FR (PARIS)	hostInstitution	2˙194˙195.00

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 Obiettivo del progetto (Objective)

'An impressive variety of motile and morphogenetic processes are driven by site-directed polarized asssembly of actin filaments. In the past ten years, breathtaking advances coming from cell biology, cell biophysics, and biochemistry have brought insight into the molecular bases for production of force and movement by site-directed actin polymerization. Yet, we do not know, with the detail sufficient to understand how force is produced, by which molecular mechanisms the filaments are nucleated or created by branching. We do not know by which elementary steps insertional polymerization of barbed ends of filaments against the membrane is performed by different protein machineries, nor how these machineries work in a coordinated fashion. Here we propose a multiscale and interdisciplinary approach of the mechanisms used by the major actin nucleators to organize the motile response of actin. The elementary reactions involved in the processive walk of formin at the growing barbed ends of filaments and the role of ATP hydrolysis in force production will be analyzed by a combination of biochemical solution studies and physical methods using functionalized GUVs and optical tweezers. The multifunctionality of WH2 domains involved in actin sequestration, filament nucleation severing and processive elongation will be similarly examined in an interdisciplinary perspective from structural biology at atomic resolution to physics at the mesoscopic scale. Biochemical and structural methods and single molecule measurements (TIRFM) will shed light into the elementary steps and structural mechanism of filament branching. Biomimetic assays with functionalized GUVs associated with biophysical methods like FRAP or fluorescence correlation spectroscopy will elucidate how different filament initiating machineries segregate in the membrane as a consequence of their interactions with growing filaments and function in a coordinated fashion during actin-based motility.'