RNASEDYNAMICS

Spatial organization and dynamics of Escherichia coli RNA degradation machinery

Coordinatore	INSTITUTO DE TECNOLOGIA QUIMICA E BIOLOGICA - UNIVERSIDADE NOVA DE LISBOA    more  Organization address address: "Avenida da Republica, Estacao Agronomica Nacional" city: OEIRAS postcode: 2784-505 contact info Titolo: Prof. Nome: Cecilia M. Cognome: Arraiano Email: send email Telefono: + 351 21 4469547 Fax: +351 21 4469314
Nazionalità Coordinatore	Portugal [PT]
Totale costo	149˙783 €
EC contributo	149˙783 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-IEF
Funding Scheme	MC-IEF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-09-01   -   2012-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUTO DE TECNOLOGIA QUIMICA E BIOLOGICA - UNIVERSIDADE NOVA DE LISBOA  Organization address address: "Avenida da Republica, Estacao Agronomica Nacional" city: OEIRAS postcode: 2784-505 contact info Titolo: Prof. Nome: Cecilia M. Cognome: Arraiano Email: send email Telefono: + 351 21 4469547 Fax: +351 21 4469314	PT (OEIRAS)	coordinator	149˙783.60

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 Obiettivo del progetto (Objective)

'The aim of this research proposal is to investigate in detail the location and mobility of RNA degradation machinery in E. coli. RNA decay has been widely studied in bacteria but continues to reveal subtle secrets [1,2]. Recent research demonstrates that the degradosome complex forms cytoskeletal-like structures linked with the inner cell membrane [3]. Preliminary results from Cecilia Arraiano’s laboratory (host laboratory) have shown that RNase R colocalises with the bacterial nucleoid, whereas RNase II is exclusively a cytoplasmic protein. Consequently, the main bacterial nucleases may have different cellular locations. After developing a system for in vivo localisation of RNases I will investigate their dynamics, likely factors that influence their mobility, and consequences of differential location. Because environmental stresses alter levels of RNases, I will check whether rearrangement of the degradation machinery is connected to changes in its cell location, and/or is linked to the formation of degradation foci analogous to eukaryotic processing/degradation P-bodies. This year it was reported in Science that abortive E. coli polymerase transcripts are produced in vivo during transcription initiation [4]. I will therefore investigate whether RNase R location in the nucleoid is functionally connected to degradation of these newly discovered small RNAs.'