SWI/SNF-CRCS

"Characterization of regulatory roles of SWI/SNF-type chromatin remodelling complexes in the control of development and hormonal, biotic and abiotic stress responses in Arabidopsis"

Coordinatore	INSTYTUT BIOCHEMII I BIOFIZYKI POLSKIEJ AKADEMII NAUK    more  Organization address address: PAWINSKIEGO 5A city: WARSZAWA postcode: 02 106 contact info Titolo: Ms. Nome: Katarzyna Cognome: Jagiello-Wilgat Email: send email Telefono: +48 22 5922141 Fax: +48 22 5922190
Nazionalità Coordinatore	Poland [PL]
Totale costo	45˙000 €
EC contributo	45˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-ERG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-09-01   -   2013-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTYTUT BIOCHEMII I BIOFIZYKI POLSKIEJ AKADEMII NAUK  Organization address address: PAWINSKIEGO 5A city: WARSZAWA postcode: 02 106 contact info Titolo: Ms. Nome: Katarzyna Cognome: Jagiello-Wilgat Email: send email Telefono: +48 22 5922141 Fax: +48 22 5922190	PL (WARSZAWA)	coordinator	45˙000.00

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Chromatin remodelling complexes (CRCs) of the SWI/SNF family are implicated in numerous regulatory processes of eukaryotic growth, differentiation and development. Multiplicity of genes encoding SNF2-type ATPase and SWI3-type SWI/SNF CRC subunits in Arabidopsis suggests that CRCs carry different combinations of these core components. In yeast two-hybrid screens, we have identified potential auxiliary proteins of Arabidopsis SWI/SNF CRCs and analyse their in vivo interactions with CRC core components by FRET assays. We also accomplished subcellular localization of core CRC subunits by in vivo imaging and established a powerful technology for purification of SWI/SNF CRCs. Our results indicate that the BRM ATPase and ATSWI3C CRC subunits occur in common SWI/SNF complexes and mutations inactivating their genes cause similar phenotypic changes. By Affymetrix transcript profiling and qRT-PCR studies, we have identified a set of genes, which show similarly altered transcription in the atswi3c and atbrm mutants. Furthermore, we characterized genetic interactions of atswi3c mutations with known mutations that affect the functions of key genes controlling physiological and developmental responses to different hormones, and stresses. The proposed reintegration project is based on a focused multidisciplinary approach aiming at precise definition of regulatory functions of SWI/SNF CRCs. Complementary tasks of the experimental work plan aim at the i) identification of primary regulatory targets of SWI/SNF CRCs using transcriptome analysis in combination with inducible knock-down SWI3 subunits; ii) mapping of SWI/SNF-binding domains in target genes using chromatin precipitation; iii) analysis of SWI/SNF CRC-regulated functions in stresses and pathogen signalling pathways. The project is expected to provide a novel insight into regulatory mechanisms by which distinct classes of SWI/SNF CRCs participate in the control of physiological and developmental responses in Arabidopsis.'