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TiFuN

Tiny Functional Au Nanorods: Novel NIR-Photothermal Nanoprobes for Single-Molecule Tracking at Confined Cellular Environment

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 Outcomes and results

 TiFuN project word cloud

Explore the words cloud of the TiFuN project. It provides you a very rough idea of what is the project "TiFuN" about.

gradient    interdisciplinary    dr    nanoprobes    elongated    dynamics    nm    setup    correlative    utilize    absorption    neuroscience    photothermal    light    photostability    haystack    prof    laser    core    group    individual    bioconjuagted    bioconjugated    dgu    size    molecule    receptors    exceptional    lounis    nanoprobe    neural    limits    density    cellular    centrifugation    choquet    spr    small    window    labeling    resonance    subsequently    multicolor    brilliant    smaller    detection    gold    regions    expertise    sensitivity    world    harvested    synapses    confined    length    detected    electron    diameter    synapse    surface    volume    ultrahigh    single    nps    probes    centered    tiny    sites    easily    combination    spectral    organelles    valuable    nanorods    domain    neurotransmitter    tackle    nir    owing    optics    proteins    specificity    chemistry    tunable    ratio    530    technique    microscopy    imaging    phi    adhesion    tifun    integrin    property    sources    lt    protocol    rods    np    cell    plasmon    needle    biology    display    tool    signal    background    nanoparticles    toxicity    nanochemistry    fellow   

Project "TiFuN" data sheet

The following table provides information about the project.

Coordinator		 CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country France [FR]
 Project website http://onlinelibrary.wiley.com/doi/10.1002/advs.201600280/epdf
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2017-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Photothermal Imaging (PhI) is an absorption detection technique used for the detection of single gold nanoparticles (NPs), which can be a valuable tool to tackle complex cellular activities. In order to study the dynamics of individual proteins in confined regions such as in neural synapses or in cell adhesion sites, small nanoprobes are needed. Gold NPs (~ 5 nm) can be used, however their surface plasmon resonance (SPR) is centered at 530 nm, a spectral domain where absorption of cellular organelles results in a background signal, which limits the sensitivity of PhI. A brilliant way to find the needle in the haystack is the development of a novel nanoprobe having NIR-absorption, smaller size and high specificity. In “TiFuN”, we will achieve this goal by developing tiny (length/diameter <10 nm/5 nm) gold nanorods (T-rods), an elongated gold NP with tunable SPR in the NIR-window. Different aspect ratio T-rods harvested using Density Gradient Ultrahigh (DGU) centrifugation protocol will be highly promising probes for multicolor labeling and detection of proteins. A dedicated PhI setup based on NIR-laser sources will also be built. Subsequently, the bioconjuagted T-rods and NIR-PhI will be used to study the dynamics of neurotransmitter receptors in synapse and integrin proteins in cell adhesion sites owing to their small core volume, NIR-absorption, exceptional photostability, and non-toxicity. Bioconjugated T-rods will also display the unique property of being easily detected with light and electron microscopy at the single- molecule level, and therefore will be a valuable tool for light/electron correlative microscopy. This highly interdisciplinary project (optics, chemistry and biology) will utilize the fellow’s expertise in nanochemistry in combination with world-leading expertise of Prof. Lounis group in single-molecule detection and Dr. Choquet institute in neuroscience and cell biology.

 Publications

year authors and title journal last update
List of publications.
2017 Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis
Small Gold Nanorods with Tunable Absorption for Photothermal Microscopy in Cells
published pages: 1600280, ISSN: 2198-3844, DOI: 10.1002/advs.201600280 		Advanced Science 4/2 2019-07-23

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