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IMpaCT SIGNED

Imaging life from Molecules to cells - building knowledge on Cryo-electron microscopy methodologies

Total Cost €

EC-Contrib. €

Partnership

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IMpaCT project word cloud

Explore the words cloud of the IMpaCT project. It provides you a very rough idea of what is the project "IMpaCT" about.

spain spanish tomography complemented visibility roles infections council devoted outcome atomic rational cells extremely 3d candidates communication crystallize workshops potentiated molecules experts forming grounded meetings science players leverage variety team dysfunction electron em prize enabled unl resolution hard symposium industry winners renowned microscopy transfer health life helsinki visits human national structural context structures nova hands antiviral labs finland weizmann cancer isolated engagement blocks seminars proteins excellent communications near israel solid expertise itqb participation workshop imaging groups wp invited centre cellular university cryo unprecedented fundamental mini nobel stage stakeholders individual international pathologies complexes macromolecular building cell plan drug portugal structure biotechnology publications

Project "IMpaCT" data sheet

The following table provides information about the project.

Coordinator	UNIVERSIDADE NOVA DE LISBOA Organization address address: CAMPUS DE CAMPOLIDE city: LISBOA postcode: 1099 085 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Portugal [PT]
Total cost	800˙000 €
EC max contribution	800˙000 € (100%)
Programme	1. H2020-EU.4.b. (Twinning of research institutions)
Code Call	H2020-WIDESPREAD-2018-03
Funding Scheme	CSA
Starting year	2019
Duration (year-month-day)	from 2019-09-01 to 2022-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	UNIVERSIDADE NOVA DE LISBOA UNIVERSIDADE NOVA DE LISBOA Organization address address: CAMPUS DE CAMPOLIDE city: LISBOA postcode: 1099 085 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	PT (LISBOA)	coordinator	412˙616.00
2	HELSINGIN YLIOPISTO HELSINGIN YLIOPISTO Organization address address: YLIOPISTONKATU 3 city: HELSINGIN YLIOPISTO postcode: 14 website: www.helsinki.fi contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FI (HELSINGIN YLIOPISTO)	participant	146˙316.00
3	WEIZMANN INSTITUTE OF SCIENCE WEIZMANN INSTITUTE OF SCIENCE Organization address address: HERZL STREET 234 city: REHOVOT postcode: 7610001 website: www.weizmann.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	IL (REHOVOT)	participant	141˙043.00
4	AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS Organization address address: CALLE SERRANO 117 city: MADRID postcode: 28006 website: http://www.csic.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	ES (MADRID)	participant	100˙023.00

Map

Project objective

Proteins are building blocks of life, often forming complexes with essential cellular roles. Dysfunction of proteins or their complexes in cells are associated with a wide variety of pathologies, making them excellent drug target candidates. Their 3D structures are essential for a rational drug design. However, many important proteins and complexes are extremely hard to crystallize. Recent advances in Cryo-Electron Microscopy methodologies enabled the structure determination of large macromolecular complexes at near-atomic resolution. An unprecedented level of detail in cell imaging using Cryo-Electron Tomography is also now possible. IMpaCT will improve the expertise at the UNL in Cryo-EM methodologies applied to the structural studies of proteins related to human health (e.g. cancer, antiviral infections) from individual molecules to macromolecular complexes, both isolated and in a cellular context. To this end, UNL will team up with renowned Cryo-EM experts from the National Centre for Biotechnology of the Spanish National Research Council (Spain), the Institute of Biotechnology of the University of Helsinki (Finland) and the Weizmann Institute of Science (Israel). A solid work programme grounded on hands-on Workshops will be complemented with short-term visits to the partner labs, visits from partner labs experts to ITQB NOVA, seminars by leading experts in Cryo-EM (e.g. Nobel Prize winners), industry stakeholders’ engagement via a Mini-symposium and workshop participation, knowledge transfer to other research groups in Portugal and a WP devoted to early-stage researchers. An ambitious Dissemination, Exploitation and Communication plan will be implemented to leverage the research results potentiated by this project as high-impact publications and invited communications at international meetings, leading to a fundamental outcome: an increased visibility and activity of UNL researchers as players in the Cryo-EM field.

Publications

List of publications.
year	authors and title	journal	last update
2017	MÃ¡rcia Alves, Nicole S.M. Vieira, LuÃs Paulo N. Rebelo, JoÃ£o M.M. AraÃºjo, Ana B. Pereiro, Margarida Archer Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme published pages: 309-320, ISSN: 0378-5173, DOI: 10.1016/j.ijpharm.2017.05.002	International Journal of Pharmaceutics 526/1-2	2019-10-30
2019	Maria C. Martins, CÃ©lia V. RomÃ£o, Filipe Folgosa, PatrÃcia T. Borges, Carlos FrazÃ£o, Miguel Teixeira How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes published pages: 36-60, ISSN: 0891-5849, DOI: 10.1016/j.freeradbiomed.2019.01.051	Free Radical Biology and Medicine 140	2019-10-30
2018	SaÃºl Silva, Osvaldo S. Ascenso, Eva C. LourenÃ§o, Margarida Archer, Christopher D. Maycock, M. Rita Ventura Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos published pages: 6860-6864, ISSN: 1477-0520, DOI: 10.1039/c8ob02096e	Organic & Biomolecular Chemistry 16/38	2019-10-30
2019	InÃªs B. Trindade, JosÃ© M. Silva, Bruno M. Fonseca, Teresa Catarino, Masaki Fujita, Pedro M. Matias, Elin Moe, Ricardo O. Louro Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility published pages: 157-167, ISSN: 0021-9258, DOI: 10.1074/jbc.ra118.005041	Journal of Biological Chemistry 294/1	2019-10-30
2017	Thomas L. Clarke, Maria Pilar Sanchez-Bailon, Kelly Chiang, John J. Reynolds, Joaquin Herrero-Ruiz, Tiago M. Bandeiras, Pedro M. Matias, Sarah L. Maslen, J. Mark Skehel, Grant S. Stewart, Clare C. Davies PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination published pages: 900-916.e7, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2017.01.019	Molecular Cell 65/5	2019-10-30
2018	Alessandro GiuffrÃ¨, JoÃ£o B. Vicente Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology published pages: 1-31, ISSN: 1942-0900, DOI: 10.1155/2018/6290931	Oxidative Medicine and Cellular Longevity 2018	2019-10-30
2019	Karim Zuhra, Pedro M. F. Sousa, Giulia Paulini, Ana Rita Lemos, Zenta Kalme, Imants Bisenieks, Egils Bisenieks, Brigita Vigante, Gunars Duburs, Tiago M. Bandeiras, Luciano Saso, Alessandro GiuffrÃ¨, JoÃ£o B. Vicente Screening Pyridine Derivatives against Human Hydrogen Sulfide-synthesizing Enzymes by Orthogonal Methods published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-018-36994-w	Scientific Reports 9/1	2019-10-30
2018	Elin Moe, Filipe Rollo, CÃ©lia M. Silveira, Murat Sezer, Peter Hildebrandt, Smilja Todorovic Spectroelectrochemical insights into structural and redox properties of immobilized endonuclease III and its catalytically inactive mutant published pages: 149-154, ISSN: 1386-1425, DOI: 10.1016/j.saa.2017.06.050	Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 188	2019-10-30
2019	Karim Zuhra, Catarina S. TomÃ©, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca MalagrinÃ², Elena Forte, JoÃ£o B. Vicente, Alessandro GiuffrÃ¨ N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells published pages: 828, ISSN: 2073-4409, DOI: 10.3390/cells8080828	Cells 8/8	2019-10-30
2019	Francesca MalagrinÃ², Karim Zuhra, Ludovica Mascolo, Daniela Mastronicola, JoÃ£o B. Vicente, Elena Forte, Alessandro GiuffrÃ¨ Hydrogen Sulfide Oxidation: Adaptive Changes in Mitochondria of SW480 Colorectal Cancer Cells upon Exposure to Hypoxia published pages: 1-11, ISSN: 1942-0900, DOI: 10.1155/2019/8102936	Oxidative Medicine and Cellular Longevity 2019	2019-10-30
2019	SÃ³nia Zacarias, Adriana TemporÃ£o, Melisa del Barrio, Vincent Fourmond, Christophe LÃ©ger, Pedro M. Matias, InÃªs A. C. Pereira A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase published pages: 8509-8519, ISSN: 2155-5435, DOI: 10.1021/acscatal.9b02347	ACS Catalysis 9/9	2019-10-30
2016	JoÃ£o B. Vicente, Francesca MalagrinÃ², Marzia Arese, Elena Forte, Paolo Sarti, Alessandro GiuffrÃ¨ Bioenergetic relevance of hydrogen sulfide and the interplay between gasotransmitters at human cystathionine Î²-synthase published pages: 1127-1138, ISSN: 0005-2728, DOI: 10.1016/j.bbabio.2016.03.030	Biochimica et Biophysica Acta (BBA) - Bioenergetics 1857/8	2019-10-30
2019	Aili Sarre, Meike Stelter, Filipe Rollo, Salvatore De Bonis, Anna Seck, CÃ©cilia Hognon, Jean-Luc Ravanat, Antonio Monari, FranÃ§ois Dehez, Elin Moe, Joanna Timmins The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities published pages: 45-59, ISSN: 1568-7864, DOI: 10.1016/j.dnarep.2019.03.014	DNA Repair 78	2019-10-30
2016	Eduardo F. P. Ruivo, LÃdia M. GonÃ§alves, LuÃs A. R. Carvalho, Rita C. Guedes, Stefan Hofbauer, JosÃ© A. Brito, Margarida Archer, Rui Moreira, Susana D. Lucas Clickable 4-Oxo-Î²-lactam-Based Selective Probing for Human Neutrophil Elastase Related Proteomes published pages: 2037-2042, ISSN: 1860-7179, DOI: 10.1002/cmdc.201600258	ChemMedChem 11/18	2019-10-30
2017	M. Amaral, D. B. Kokh, J. Bomke, A. Wegener, H. P. Buchstaller, H. M. Eggenweiler, P. Matias, C. Sirrenberg, R. C. Wade, M. Frech Protein conformational flexibility modulates kinetics and thermodynamics of drug binding published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-02258-w	Nature Communications 8/1	2019-10-30
2016	CÃ©lia V. RomÃ£o, JoÃ£o B. Vicente, PatrÃcia T. Borges, Bruno L. Victor, Pedro Lamosa, ElÃsio Silva, LuÃs Pereira, Tiago M. Bandeiras, ClÃ¡udio M. Soares, Maria A. Carrondo, David Turner, Miguel Teixeira, Carlos FrazÃ£o Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase published pages: 4686-4707, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2016.10.008	Journal of Molecular Biology 428/23	2019-10-30
2019	Bruno M. Fonseca, LuÃs Silva, InÃªs B. Trindade, Elin Moe, Pedro M. Matias, Ricardo O. Louro, Catarina M. Paquete Optimizing Electroactive Organisms: The Effect of Orthologous Proteins published pages: , ISSN: 2296-598X, DOI: 10.3389/fenrg.2019.00002	Frontiers in Energy Research 7	2019-10-30
2018	CÃ©lia V. RomÃ£o, Pedro M. Matias, Cristiana M. Sousa, Filipa G. Pinho, Ana F. Pinto, Miguel Teixeira, Tiago M. Bandeiras Insights into the Structures of Superoxide Reductases from the Symbionts Ignicoccus hospitalis and Nanoarchaeum equitans published pages: 5271-5281, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00334	Biochemistry 57/36	2019-10-30
2016	InÃªs B. Trindade, Bruno M. Fonseca, Pedro M. Matias, Ricardo O. Louro, Elin Moe A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB 400: cloning, expression, purification, crystallization and X-ray diffraction analysis published pages: 667-671, ISSN: 2053-230X, DOI: 10.1107/s2053230x16011419	Acta Crystallographica Section F Structural Biology Communications 72/9	2019-10-30
2017	Luis Pereira, Ivo H. Saraiva, A. Sofia F. Oliveira, ClÃ¡udio M. Soares, Ricardo O. Louro, Carlos FrazÃ£o Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2 published pages: 847-853, ISSN: 0005-2728, DOI: 10.1016/j.bbabio.2017.07.002	Biochimica et Biophysica Acta (BBA) - Bioenergetics 1858/10	2019-10-30
2018	Sara T. N. Silva, JosÃ© A. Brito, RocÃo Arranz, Carlos Ã“scar S. Sorzano, Christine Ebel, James Doutch, Mark D. Tully, JosÃ©-MarÃa Carazo, JosÃ© L. Carrascosa, Pedro M. Matias, Tiago M. Bandeiras X-ray structure of full-length human RuvB-Like 2 â€“ mechanistic insights into coupling between ATP binding and mechanical action published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-018-31997-z	Scientific Reports 8/1	2019-10-30
2017	Filipe M. Sousa, Filipa V. Sena, Ana P. Batista, Diogo Athayde, JosÃ© A. Brito, Margarida Archer, A. Sofia F. Oliveira, ClÃ¡udio M. Soares, Teresa Catarino, Manuela M. Pereira The key role of glutamate 172 in the mechanism of type II NADH:quinone oxidoreductase of Staphylococcus aureus published pages: 823-832, ISSN: 0005-2728, DOI: 10.1016/j.bbabio.2017.08.002	Biochimica et Biophysica Acta (BBA) - Bioenergetics 1858/10	2019-10-30
2019	PatrÃcia T. Borges, CÃ©lia V. RomÃ£o, LÃgia M. Saraiva, Vera L. GonÃ§alves, Maria A. Carrondo, Miguel Teixeira, Carlos FrazÃ£o Analysis of a new flavodiiron core structural arrangement in Flv1-Î”FlR protein from Synechocystis sp. PCC6803 published pages: 91-102, ISSN: 1047-8477, DOI: 10.1016/j.jsb.2018.11.004	Journal of Structural Biology 205/1	2019-10-30
2017	Ana I. Coelho, M. Estela Rubio-Gozalbo, JoÃ£o B. Vicente, Isabel Rivera Sweet and sour: an update on classic galactosemia published pages: 325-342, ISSN: 0141-8955, DOI: 10.1007/s10545-017-0029-3	Journal of Inherited Metabolic Disease 40/3	2019-10-30
2016	Susana A.L. Lobo, Marco A.M. Videira, Isabel Pacheco, Mark N. Wass, Martin J. Warren, Miguel Teixeira, Pedro M. Matias, CÃ©lia V. RomÃ£o, LÃgia M. Saraiva Desulfovibrio vulgaris CbiK P cobaltochelatase: evolution of a haem binding protein orchestrated by the incorporation of two histidine residues published pages: 106-118, ISSN: 1462-2912, DOI: 10.1111/1462-2920.13479	Environmental Microbiology 19/1	2019-10-30
2016	Joanna Timmins, Elin Moe A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges published pages: 168-176, ISSN: 2001-0370, DOI: 10.1016/j.csbj.2016.04.001	Computational and Structural Biotechnology Journal 14	2019-10-30
2017	Marta C Marques, Cristina Tapia, Oscar GutiÃ©rrez-Sanz, Ana Raquel Ramos, Kimberly L Keller, Judy D Wall, Antonio L De Lacey, Pedro M Matias, InÃªs A C Pereira The direct role of selenocysteine in [NiFeSe] hydrogenase maturation and catalysis published pages: 544-550, ISSN: 1552-4450, DOI: 10.1038/nchembio.2335	Nature Chemical Biology 13/5	2019-10-30
2017	Sandra P. Santos, Maxime G. Cuypers, Adam Round, Stephanie Finet, Theyencheri Narayanan, Edward P. Mitchell, CÃ©lia V. RomÃ£o SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions published pages: 667-687, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2017.01.008	Journal of Molecular Biology 429/5	2019-10-30
2016	Julia M. Kurth, JosÃ© A. Brito, Jula Reuter, Alexander Flegler, Tobias Koch, Thomas Franke, Eva-Maria Klein, Sam F. Rowe, Julea N. Butt, Kevin Denkmann, InÃªs A. C. Pereira, Margarida Archer, Christiane Dahl Electron Accepting Units of the Diheme Cytochrome c TsdA, a Bifunctional Thiosulfate Dehydrogenase/Tetrathionate Reductase published pages: 24804-24818, ISSN: 0021-9258, DOI: 10.1074/jbc.m116.753863	Journal of Biological Chemistry 291/48	2019-10-30
2017	JoÃ£o B. Vicente, Henrique G. ColaÃ§o, Francesca MalagrinÃ², Paulo E. Santo, AndrÃ© Gutierres, Tiago M. Bandeiras, Paula Leandro, JosÃ© A. Brito, Alessandro GiuffrÃ¨ A Clinically Relevant Variant of the Human Hydrogen Sulfide-Synthesizing Enzyme Cystathionine Î² -Synthase: Increased CO Reactivity as a Novel Molecular Mechanism of Pathogenicity? published pages: 1-13, ISSN: 1942-0900, DOI: 10.1155/2017/8940321	Oxidative Medicine and Cellular Longevity 2017	2019-10-30
2018	Kelly Stefany Tuna Frade, Andreia CecÃlia Pimenta Fernandes, Celia Marisa Silveira, Carlos FrazÃ£o, Elin Moe A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans : cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis published pages: 419-424, ISSN: 2053-230X, DOI: 10.1107/s2053230x18008488	Acta Crystallographica Section F Structural Biology Communications 74/7	2019-10-30
2018	M. CecÃlia Almadanim, Nuno M. GonÃ§alves, Margarida T.G. Rosa, Bruno M. Alexandre, AndrÃ© M. Cordeiro, Mafalda Rodrigues, Nelson J.M. Saibo, ClÃ¡udio M. Soares, CÃ©lia V. RomÃ£o, M. Margarida Oliveira, Isabel A. Abreu The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications published pages: 231-246, ISSN: 0167-4889, DOI: 10.1016/j.bbamcr.2017.10.010	Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1865/2	2019-10-30

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