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3DQuant

Understanding long-range transcriptional regulation in the context of the 3D genome organization

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 3DQuant project word cloud

Explore the words cloud of the 3DQuant project. It provides you a very rough idea of what is the project "3DQuant" about.

action    expression    generate    measuring    isolated    associating    genes    suggests    loops    conformation    partitioning    space    cell    quantitatively    correlated    3d    promoter    engineered    sub    single    folded    self    quantitative    regulation    enhancer    stem    modulates    cognate    regulatory    structure    distances    domains    linked    relationship    chromatin    genomic    gene    patterns    communication    tads    contributes    partitioned    close    influences    genetic    unprecedented    mammalian    environment    embryonic    measured    mouse    temporal    capture    manner    mechanisms    physical    biophysical    unknown    live    mediated    transcriptional    technologies    enhancers    imaging    dimensional    architecture    modulation    pairs    spatial    cells    mechanism    genome    engineering    view    proximity    organization    interactions    unravel    establishment    transcription    promoters    3c    mobilized    chromosome    topologically    shown    metazoans    chromosomes    precisely    modulate    outputs    techniques    underlying    megabase   

Project "3DQuant" data sheet

The following table provides information about the project.

Coordinator
FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION 

Organization address
address: MAULBEERSTRASSE 66
city: BASEL
postcode: 4058
website: www.fmi.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 175˙419 €
 EC max contribution 175˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH FONDATION CH (BASEL) coordinator 175˙419.00

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 Project objective

Enhancers are regulatory elements that control the spatial and temporal expression of genes in metazoans. Enhancers are able to modulate transcription of a target gene from large genomic distances, as a result of the formation of chromatin loops that bring them in close spatial proximity to cognate promoters. The manner how specific patterns of enhancer-promoter physical interactions are established is linked to how chromosomes are folded in the three-dimensional (3D) space. Recent studies based on chromosome conformation capture (3C) have shown that mammalian chromosomes are partitioned into self-associating sub-megabase domains called Topologically Associating Domains (TADs). Genetic evidence suggests that 3D chromatin organization within and across TADs contributes to the establishment and partitioning of enhancers-promoters physical communication. Yet it is still unknown by which biophysical mechanisms chromosome architecture modulates enhancer action, and thus transcription. The goal of this proposal is to determine the quantitative relationship between 3D chromatin architecture and enhancer-promoter activity to unravel the mechanism of long-range transcriptional modulation mediated by enhancers. Addressing this goal requires a system where transcriptional outputs can be measured precisely and quantitatively, and correlated with 3D distances. To this aim, we will use state-of-the art genome engineering techniques to generate mouse embryonic stem cells with engineered enhancer-promoter pairs in an isolated chromatin environment, where a selected enhancer can be mobilized at different distances from its cognate promoter. We will use this system to quantitatively assess how 3D chromatin structure influences enhancer action by measuring transcription and promoter-enhancer interactions using 3C-based technologies, single-cell methods and live-cell imaging. This will lead to an unprecedented view of the mechanisms underlying long-range transcriptional regulation.

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