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HITSCIL SIGNED

How intraflagellar transport shapes the cilium: a single-molecule systems study

Total Cost €

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EC-Contrib. €

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Partnership

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 HITSCIL project word cloud

Explore the words cloud of the HITSCIL project. It provides you a very rough idea of what is the project "HITSCIL" about.

experiments    external    dynamically    consist    structure    maintenance    antennas    proteins    regulated    specialized    cilia    ciliary    ift    place    molecules    connection    visualize    solutions    quantify    disturbances    dynamics    sensing    environment    organelle    lab    shed    substantially    intraflagellar    microtubule    eukaryotic    organelles    signalling    driving    single    indicated    chemosensory    quantitative    mechanism    exposure    zoom    vice    fundamental    function    laboratory    position    fascinating    perturbations    chemotaxis    probes    directional    nbsp    soluble    fluorescent    light    elegans    transport    animals    relationship    intracellular    functions    membrane    sensory    sensors    understand    repellent    toolbox    perspective    critically    preliminary    shown    microscopy    versa    sense    cooperation    water    microtubules    living    tools    fluorescence    enveloped    molecule    cells    instrumentation    push    animal    length    axoneme    motor    unprecedented    axonemal   

Project "HITSCIL" data sheet

The following table provides information about the project.

Coordinator
STICHTING VU 

Organization address
address: DE BOELELAAN 1105
city: AMSTERDAM
postcode: 1081 HV
website: www.vu.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙499˙580 €
 EC max contribution 2˙499˙580 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2023-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    STICHTING VU NL (AMSTERDAM) coordinator 2˙499˙580.00

Map

 Project objective

Sensory cilia are organelles extending like antennas from many eukaryotic cells, with crucial functions in sensing and signalling. Cilia consist of an axoneme built of microtubules, enveloped by a specialized membrane. Ciliary development and maintenance depend critically on a specific, microtubule-based intracellular transport mechanism, intraflagellar transport (IFT). In my laboratory, we study the chemosensory cilia of C. elegans, which sense water-soluble molecules in the animal’s environment for chemotaxis. Over the past years, we have developed a unique set of quantitative, single-molecule fluorescence microscopy tools that allow us to visualize and quantify IFT dynamics with unprecedented detail in living animals. So far, our focus has been on the cooperation of the motor proteins driving IFT. The overall objective of my current proposal is to zoom out and shed light on the connection between ciliary structure, chemosensory function and IFT, from a systems perspective. Recent work has indicated that axoneme length is controlled by IFT. Preliminary results from my laboratory show that axoneme length changes dynamically in response to perturbations of IFT or cilia. Furthermore, we have shown that IFT is substantially affected upon exposure of animals to known repellent solutions. The four major aims in my proposal are to: • determine how directional changes in IFT are regulated and are affected by external disturbances, • understand the dynamics of the axonemal microtubules and how IFT affects these dynamics and vice versa, • study how sensory ciliary function affects IFT and ciliary structure, • further develop our (single-molecule) fluorescence microscopy toolbox by improving instrumentation and using better fluorescent probes and sensors. These experiments will place my lab in a unique position to push forward our understanding of the relationship between structure, function and dynamics of transport of this fascinating and fundamental organelle.

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The information about "HITSCIL" are provided by the European Opendata Portal: CORDIS opendata.

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