FRT-HOMING

Identifying new Markers and Homing Profiles involved in Lymphocyte Migration to the Female Reproductive Tract

Coordinatore	INSTITUT DE INVESTIGACIO EN CIENCIES DE LA SALUT GERMANS TRIAS I PUJOL    more  Organization address address: Carretera de Canyet s/n city: BADALONA BARCELONA postcode: 8916 contact info Titolo: Dr. Nome: Manuel Cognome: Puig-Domingo Email: send email Telefono: +34 93 497 8650 Fax: +34 93 497 86 54
Nazionalità Coordinatore	Spain [ES]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-CIG
Funding Scheme	MC-CIG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-04-01   -   2016-09-17

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT DE INVESTIGACIO EN CIENCIES DE LA SALUT GERMANS TRIAS I PUJOL  Organization address address: Carretera de Canyet s/n city: BADALONA BARCELONA postcode: 8916 contact info Titolo: Dr. Nome: Manuel Cognome: Puig-Domingo Email: send email Telefono: +34 93 497 8650 Fax: +34 93 497 86 54	ES (BADALONA BARCELONA)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'The effort to develop vaccines that can elicit mucosal immune responses in the female reproductive tract (FRT) against sexually transmitted infections (STI) have been hampered by an inability to accurately measure immune responses in the genital tract. Vaccines against STIs should generate localized memory responses at sites of potential exposure to provide better control of infection. Critically, lymphocytes isolated from the genital tract will migrate back into the FRT when transferred into a naïve recipient. Yet, the repertoire of trafficking molecules expressed on cells that will home to FRT is still unknown. The hypothesis of this project is that lymphocytes induced by FRT immunization traffic transiently in blood expressing a specific set of homing markers, but are selectively retained in the FRT after transmigration. By isolating cells from blood shortly after mucosal FRT immunization in animal models and FRT-mucosal infection in patients, we will be able to detect the potential specific integrins and homing receptors responsible for lymphocyte entry into the FRT. The goal is to identify specific integrins and homing receptors expressed on circulating T and B lymphocytes that direct their migration to the female reproductive tract during the brief period in which mucosal lymphocytes re-circulate. Direct comparison of gene and protein expression profiles using microarrays and mass spectrometry technology on subsets of purified peripheral blood will be performed in patients and murine models. Differentially expressed candidate molecules selected from these functional genomic analyses will be confirmed and validated in patients and by competitive homing assays in vivo in mice. Identifying the profile characteristic of lymphocytes migrating to the FRT soon after vaccination or infection will have a critical impact on the detection of recent exposure to a sexually transmitted pathogen and on the development of new STI vaccines.'