RETRAIN

REwiring the nucleus TowaRds plant ImmuNity

Coordinatore	UNIVERSITY OF DUNDEE    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	1˙393˙084 €
EC contributo	1˙393˙084 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2012-StG_20111109
Funding Scheme	ERC-SG
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-01-01   -   2017-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITY OF DUNDEE  Organization address address: Nethergate city: DUNDEE postcode: DD1 4HN contact info Titolo: Dr. Nome: Edgar Cognome: Huitema Email: send email Telefono: 441383000000	UK (DUNDEE)	hostInstitution	1˙393˙084.00
2	UNIVERSITY OF DUNDEE  Organization address address: Nethergate city: DUNDEE postcode: DD1 4HN contact info Titolo: Dr. Nome: Kathryn Cognome: Williamson Email: send email Telefono: +44 1382 388340	UK (DUNDEE)	hostInstitution	1˙393˙084.00

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 Obiettivo del progetto (Objective)

'Plant pathogenic microbes cause significant economic damage to global food crop production systems. Pathogens are specialised microbes that manipulate the host defense circuitry to suppress immunity and promote virulence. Interestingly, many of the processes that determine immunity or susceptible interactions, take place in the host nucleus. With the identification of vast repertoires of (nuclear) secreted pathogen proteins (effectors) and host signaling components that extert their functions in the nucleus, this host compartment is now considered an important interface where outcomes are decided.

This proposal aims to unravel the molecular mechanisms that underpin infection outcomes in the nucleus. A powerfull approach that will combine quantitative proteomics with gene expression analyses will be used to (i) detect pathogen effectors in the host nucleus in situ and (ii) identify changes in the nuclear proteome during infection. I will then use Yeast two Hybrid and immunoprecipitation to identify effector host targets before placing these components in a nuclear immune signalling context. New information on effector-induced changes in the host nuclear proteome coupled to immune signalling, will then be used to rationalise implementation of two synthetic approaches towards resistance.

I will design synthetic (non)host targets that are immune to effector induced modifications and function towards immunity. In addition, identification of key effectors and targets will be used to design new R-gene like specificities, embodied in synthetic Nucleotide-Binding Leucine Rich Repeat proteins (sNB-LRR). Overall, this work will impact studies on host-microbe interactions and importantly, will provide proof of concept for two new synthetic breeding strategies in plants.'