T6SS-PSEUDO-EFFECTOR
Identification of novel effectors of the Type Six Secretion Systems in Pseudomonas aeruginosa
| Coordinatore |
Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD contact info |
| Nazionalità Coordinatore | Non specificata |
| Totale costo | 221˙606 € |
| EC contributo | 221˙606 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2012-IIF |
| Anno di inizio | 2014 |
| Periodo (anno-mese-giorno) | 2014-03-01 - 2016-02-29 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE
Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD contact info |
UK (LONDON) | coordinator | 221˙606.40 |
Mappa
Word cloud
Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.
Obiettivo del progetto (Objective)
'Pseudomonas aeruginosa is a human pathogen causing life-threatening nosocomial infections. Its virulence mechanisms are pleiotropic, but secretion systems have a key role in transporting effectors, toxins and other virulence factors from the bacteria into the environment or target host cells. In recent years, a novel secretion system, the Type VI secretion system (T6SS), was shown to be important for P. aeruginosa virulence. Of three T6SSs encoded on the P. aeruginosa genome, the H1-T6SS is important for P. aeruginosa to compete with other bacteria and to establish chronic infections. H1-T6SS is involved in the secretion of at least three bacteriolytic effectors, Tse1-3, whereas no effectors have been identified for H2- and H3-T6SS. We hypothesise that proteins secreted by these two systems mediate other important aspects of P. aeruginosa virulence and their identification and characterisation are crucial to further understanding the H2- and H3-T6SS function and P. aeruginosa pathogenesis. This project will identify these effectors, using three approaches: 1) identification of regulatory elements controlling H2- and H3-T6SS gene expression in order to up-regulate expression of the H2- or H3-T6SS clusters and other co-regulated genes; 2) analysis of H1-, H2- and H3-T6SS gene expression in biofilm conditions using flowcells; 3) identification of H2- and H3-T6SS secreted effector proteins via secretome analysis of strains that have up-regulated expression of H2 and H3-T6SS clusters. The discovery of these secreted factors will be a breakthrough, since only a very limited number of T6SS substrates have been identified. It will give new insights into P. aeruginosa virulence and could be exploited for development of new therapeutic options.'