3x3D Imaging

Fast two-photon in vivo imaging and stimulation with simultaneous three-dimensional random-access scanning in multiple brain regions

Coordinatore	FEMTONICS KUTATO ES FEJLESZTO KORLATOLT FELELOSSEGU TARSASAG    more  Organization address address: TUZOLTO UTCA 59 city: BUDAPEST contact info Titolo: Dr. Nome: Patricia Cognome: Varju Email: send email Telefono: +36 30 4679373 Fax: +36 1 797 2348
Nazionalità Coordinatore	Hungary [HU]
Totale costo	2˙305˙232 €
EC contributo	1˙690˙999 €
Programma	FP7-ICT Specific Programme "Cooperation": Information and communication technologies
Code Call	FP7-ICT-2013-C
Funding Scheme	CP
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-06-01   -   2016-05-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	FEMTONICS KUTATO ES FEJLESZTO KORLATOLT FELELOSSEGU TARSASAG  Organization address address: TUZOLTO UTCA 59 city: BUDAPEST contact info Titolo: Dr. Nome: Patricia Cognome: Varju Email: send email Telefono: +36 30 4679373 Fax: +36 1 797 2348	HU (BUDAPEST)	coordinator	0.00
2	BUDAPESTI MUSZAKI ES GAZDASAGTUDOMANYI EGYETEM  Organization address address: MUEGYETEM RAKPART city: BUDAPEST contact info Titolo: Dr. Nome: Pál Cognome: Maák Email: send email Telefono: +36 70 2999106 Fax: +36 1 463 4195	HU (BUDAPEST)	participant	0.00
3	FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH  Organization address address: MAULBEERSTRASSE city: BASEL contact info Titolo: Mrs. Nome: Dorothy Cognome: Searles Email: send email Telefono: +41 61 6972982 Fax: +41 61 6973976	CH (BASEL)	participant	0.00
4	MAX-DELBRUCK-CENTRUM FUR MOLEKULARE MEDIZIN STIFTUNG  Organization address address: Robert-Roessle-Strasse 10 city: BERLIN contact info Titolo: Dr. Nome: Ioannis Cognome: Legouras Email: send email Telefono: +49 30 9406 4247 Fax: +49 30 94062700	DE (BERLIN)	participant	0.00
5	PAZMANY PETER KATOLIKUS EGYETEM  Organization address address: Szentkiralyi u. 28 city: BUDAPEST contact info Titolo: Mrs. Nome: Erika Cognome: Aliné Csereháti Email: send email Telefono: 3618864792	HU (BUDAPEST)	participant	0.00
6	SZEGEDI TUDOMANYEGYETEM  Organization address address: DUGONICS TER city: SZEGED contact info Titolo: Ms. Nome: Veronika Cognome: Verseghy Email: send email Telefono: +36 62 544223 Fax: +36 62 544 658	HU (SZEGED)	participant	0.00

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

We propose to engineer and validate a novel random access three-dimensional two-photon (2P) laser scanning microscope which can simultaneously image three different brain regions in the three spatial dimensions (3x3D system), where each scanned volume can exceed cubic millimeters. Our 3x3D system, which allows the simultaneous recording of neuronal activity in multiple, functionally connected distant brain volumes, is essential for understanding distributed brain computations. As each site can be scanned with sub-millisecond temporal resolution in several hundred user defined locations, the activity of single neurons in neuronal microcircuits or even neuronal compartments such as dendrites or axons will be followed. Furthermore, by combining imaging with powerful genetic methods and 3D photo-activation, we will not only record from identified circuits but also map connectivity between regions. We will validate the capability of our 3x3D system by simultaneously performing retinal, lateral geniculate nucleus, and cortical 3D imaging and photo stimulation. No currently available technology is able to achieve such multi-region 3D recordings of neuronal activity, considering not only products on the market but scientific publications as well. In the long term, the project leader SME will release a high-value added product family to the world market based on the methods and results that are realized and validated during this project. Despite steep competition from overseas laboratories, the first single region 3D 2P brain imaging was invented in Europe. Now, having similarly heavy competition this project attempts to realize the first multi-region 3D 2P brain imaging microscope.