CSKFINGERPRINTS

Mechanical loading to direct stem cell differentiation

Coordinatore	QUEEN MARY UNIVERSITY OF LONDON    more  Organization address address: 327 MILE END ROAD city: LONDON postcode: E1 4NS contact info Titolo: Mr. Nome: Greg Cognome: Dow Email: send email Telefono: +44020 7882 2569
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-CIG
Funding Scheme	MC-CIG
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-05-01   -   2018-04-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	QUEEN MARY UNIVERSITY OF LONDON  Organization address address: 327 MILE END ROAD city: LONDON postcode: E1 4NS contact info Titolo: Mr. Nome: Greg Cognome: Dow Email: send email Telefono: +44020 7882 2569	UK (LONDON)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'The main goal of this proposal is to induce and detect in real time and at single-cell level the differentiation of human mesenchymal stem cells using mechanical loads in 2D and 3D conditions. This proposal integrates disciplines from nanotechnology, bioengineering and cell and molecular biology. First we propose to develop a method to track the differentiation of stem cells in real time and at the single cell level using cytoskeletal organization of actin, microtubules and intermediate filaments as a suitable cell biomarker. We will then establish mechanical loading protocols to induce, via direct force application onto cells, the first stages of stem cell differentiation towards specific cell lineages. We will apply cyclic tensile strain and compression to stem cells in 2D and 3D conditions, and track their differentiation status in real time using the cytoskeletal biomarkers that we will have identified before. The results of this proposal will have implications for the field of stem cell mechanobiology in particular, and some of the techniques developed will also contribute to the wider field of directed stem cell differentiation.'