DIAZOASSOCRICE

Understanding the Micro-Environments of Diazotrophs and their Associated Activities in Rice

Coordinatore	UNIVERSITAT WIEN    more  Organization address address: UNIVERSITATSRING 1 city: WIEN postcode: 1010 contact info Titolo: Dr. Nome: Dagmar Cognome: Woebken Email: send email Telefono: +43 1 4277 76613 Fax: +43 1 4277 876613
Nazionalità Coordinatore	Austria [AT]
Totale costo	179˙137 €
EC contributo	179˙137 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-IEF
Funding Scheme	MC-IEF
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-08-01   -   2016-09-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITAT WIEN  Organization address address: UNIVERSITATSRING 1 city: WIEN postcode: 1010 contact info Titolo: Dr. Nome: Dagmar Cognome: Woebken Email: send email Telefono: +43 1 4277 76613 Fax: +43 1 4277 876613	AT (WIEN)	coordinator	179˙137.20

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Rice (Oryza sativa L.) is one of the world’s most important crop plants. The production is strongly limited by nitrogen (N), which is typically supplied by industrial fertilizers that are costly and hazardous to the environment. Biological nitrogen fixation (BNF) through N2 fixing Bacteria and Archaea (diazotrophs) can alleviate the N-shortage in rice cultivation and was estimated to account for up to 25% of the total N-demand of the plants. However, our knowledge on N2 fixation related processes in the soil-microbe-plant interface of flooded rice fields (paddy soils) is still rudimentary. The proposed project seeks to increase the understanding of N2 fixation rates of diazotrophs and their respective contribution to the total BNF in these systems. A better understanding of the colonization patterns and preferential micro-niches of diazotroph associated with soil-grown rice roots is needed to unravel the interactions and N-transfer processes between diazotrophs and the rice plant on a biologically meaningful micro-scale. The objectives of the proposed research are: (1) to investigate the factors (soil types, rice genotypes, plant growth stages) influencing the community composition of diazotrophs associated with different soil/root micro-environments based on nifH amplicon sequencing; (2) the in situ analysis of spatial distribution and colonization patterns of native diazotrophs associated with soil-grown rice roots via fluorescence in situ hybridization (FISH); and (3) to assess the in situ activity of diazotrophs associated with soil-root micro-environments by 15N2 incubations and FISH-NanoSIMS. This project combines molecular methods, biogeochemical assays, and single-cell isotope analysis to analyze the in situ activities of diazotrophs in a previously unachievable manner. This multidisciplinary approach will be extremely powerful to address questions regarding the N2 fixation by plant-associated diazotrophs on a biologically relevant submicron scale.'