STELUM

Spatio-Temporal Engineering of Light. Ultimate Multiphoton Microscopy

Coordinatore	more  Organization address address: AVINGUDA CARL FRIEDRICH GAUSS 3 city: Castelldefels postcode: 8860 contact info Titolo: Ms. Nome: Dolors Cognome: Mateu Email: send email Telefono: -935534048 Fax: -935533966
Nazionalità Coordinatore	Non specificata
Totale costo	524˙760 €
EC contributo	524˙760 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-3-1-IAPP
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-06-01   -   2012-05-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	FUNDACIO INSTITUT DE CIENCIES FOTONIQUES  Organization address address: AVINGUDA CARL FRIEDRICH GAUSS 3 city: Castelldefels postcode: 8860 contact info Titolo: Ms. Nome: Dolors Cognome: Mateu Email: send email Telefono: -935534048 Fax: -935533966	ES (Castelldefels)	coordinator	0.00
2	IMAGINE OPTIC  Organization address address: 18 RUE CHARLES DE GAULLE city: ORSAY postcode: 91400 contact info Titolo: Ms. Nome: Nathalie Cognome: Mandonnet Email: send email Telefono: 33164861560 Fax: 33164861561	FR (ORSAY)	participant	0.00

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 Obiettivo del progetto (Objective)

'The development of high resolution, non damaging imaging techniques are crucial for understanding the biological processes occurring at the cellular level. Nonlinear microscopy (NLM) is rapidly establishing as a powerful technique for high resolution imaging of living biological samples. The high peak powers and low pulse energies available from ultrashort pulses, allow an efficient excitation of nonlinear effects with reduced collateral damage when interacting with cells. Ultrashort pulse light has three additional parameters that can be exploited for a more efficient light-cell interaction. i) Pulse shaping, acting on the temporal intensity profile and phase parameters of a pulse will allow for a more efficient and less damaging interaction with the sample. ii) Adaptive optics will be used to modulate the beam’s wavefront spatial distribution parameter. This will allow correcting aberrations for an increased transversal resolution, larger penetration depths and fields of view. As focus spot size is reduced, it will also allow for lower power to be used, preserving living specimens. In this project, we aim, for the first time, to join complementary intersectorial expertise to perform simultaneous pulse shaping and wavefront correction at the sample plane of a NLM. The knowledge of each of the teams will be transferred, based on microscopy applications. Firstly the work will be focused towards implementing adaptive optics to minimise aberrations induced by the setup and by the sample of study. Then pulse shaping will be use to enhance the output signal and reduce side effects (such as photobleaching). Finally, the combined action will result on new contrast generation technique, constituting an important breakthrough in NLM with great implications into bio-medicine.'

Introduzione (Teaser)

Imaging biological tissue in vivo is critical to our understanding of cellular processes and to advanced diagnostics. Novel microscopy technologies developed with EU support have advanced the state - of - the - art.