SMYD3 AND MYOGENESIS

Potential role of the histonemethylase SMYD3 in myogenesis

Coordinatore	UNIVERSITA DEGLI STUDI DI MILANO    more  Organization address address: Via Festa Del Perdono 7 city: MILANO postcode: 20122 contact info Titolo: Prof. Nome: Roberto Cognome: Mantovani Email: send email Telefono: 39-02-50315005 Fax: -50315085
Nazionalità Coordinatore	Italy [IT]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-4-3-IRG
Funding Scheme	MC-IRG
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-09-03   -   2012-09-02

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITA DEGLI STUDI DI MILANO  Organization address address: Via Festa Del Perdono 7 city: MILANO postcode: 20122 contact info Titolo: Prof. Nome: Roberto Cognome: Mantovani Email: send email Telefono: 39-02-50315005 Fax: -50315085	IT (MILANO)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'SMYD3 is a histone methylase that di- and tri-methylates histone H3 at lysine 4. In normal conditions SMYD3 is highly expressed only in skeletal muscle and testis. We hypothesize that SMYD3 plays an important role in transcriptional regulation throughout myogenesis. We will use C2C12 myoblast and primary satellite cells as model systems to assess the contribution of SMYD3 to the transcriptional regulation of muscle specific genes. We will over-express and knock down SMYD3 to determine the impact of SMYD3 on the expression of myogenic markers and the ability of C2C12 cells to fuse into multinucleated myotubes. We will also determine functional mechanisms that account for a potential involvement of SMYD3 in myogenesis by employing chromatin immunoprecipitation experiments. Moreover, we will identify SMYD3 target genes in muscle cells by RNA microarray analysis and by the combination of the chromatin immunoprecipitation technique with massively sequencing technology (ChIp-seq). A further objective of the project will be to gain insights into the mechanisms underlying SMYD3 activity and its modulation, by exploiting in vitro and in vivo approaches.'