ZF OPTOGENETICS

Optogenetic analysis of neural circuits controlling behavior in zebrafish

Coordinatore	Novartis Forschungsstiftung    more  Organization address address: Maulbeerstrasse 66 city: BASEL postcode: 4058 contact info Titolo: Ms. Nome: Dorothy Cognome: Searles Email: send email Telefono: -6973002 Fax: -6973996
Nazionalità Coordinatore	Switzerland [CH]
Totale costo	177˙065 €
EC contributo	177˙065 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-IEF
Funding Scheme	MC-IEF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-07-01   -   2012-06-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	Novartis Forschungsstiftung  Organization address address: Maulbeerstrasse 66 city: BASEL postcode: 4058 contact info Titolo: Ms. Nome: Dorothy Cognome: Searles Email: send email Telefono: -6973002 Fax: -6973996	CH (BASEL)	coordinator	177˙065.20

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 Obiettivo del progetto (Objective)

'The aim of this project is to understand how behaviours are controlled by neuronal circuits in zebrafish. Genetic methods such as the Gal4-UAS system will be used to express the light-gated cation channel channelrhodopsin-2 and the light-gated chloride pump halorhodopsin in defined neuronal populations. These optogenetic tools permit the stimulation (channelrhodopsin) or inhibition (halorhodopsin) of genetically targeted neurons by light in transparent zebrafish in vivo. Effects of these manipulations on behavior will be examined in forward and reverse behavioral screens. In forward experiments, swimming behaviors evoked by uniform or patterned stimulation of neurons will be analyzed in an unbiased manner. In reverse experiments, the effect of silencing defined populations of neurons will be evaluated in the context of an olfactory discrimination learning paradigm. The identification of defined neuronal populations involved in different behaviours will then serve as the starting point for the anatomical and functional analysis of the underlying circuits. The connectivity of the identified neuronal populations will be examined by anatomical methods and transsynaptic viral tracers. The function of neuronal circuits will be analyzed using 2-photon calcium imaging and electrophysiological methods. This integrated approach is expected to provide novel insights into the principles of information processing in the brain and establish a direct relationship between neuronal circuit function and behaviour.'