HCSP IN BETA-CELLS
The highly calcium-sensitive pool of granules in biphasic insulin secretion: Experimental and theoretical investigations
| Coordinatore | LUNDS UNIVERSITET
Organization address
address: Paradisgatan 5c contact info |
| Nazionalità Coordinatore | Sweden [SE] |
| Totale costo | 136˙002 € |
| EC contributo | 136˙002 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2009-IEF |
| Funding Scheme | MC-IEF |
| Anno di inizio | 2010 |
| Periodo (anno-mese-giorno) | 2010-06-01 - 2012-01-12 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
LUNDS UNIVERSITET
Organization address
address: Paradisgatan 5c contact info |
SE (LUND) | coordinator | 136˙002.05 |
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Obiettivo del progetto (Objective)
'Insulin is released from the pancreatic beta-cells in a biphasic manner in response to a step in the ambient glucose concentration, and the loss of first-phase secretion is an early marker of diabetes. Studies have shown that there exist pools of insulin secretory granules with different calcium sensitivities. The project will study the nature, regulation and role of the highly calcium-sensitive pool (HCSP) in pancreatic beta-cells. Special focus will be on its relation to "newcomer granules", which fuse shortly after arriving to the membrane, during biphasic insulin secretion. We will apply a combination of well-established experimental methods such as patch-clamp techniques, life confocal and TIRF imaging, in novel combinations with knock-down of key exocytotic proteins, in particular Syntaxin and Synaptotagmin. The experimental part will be complemented and supported by modelling studies, building on our recently published model, which identified the HCSP with newcomer granules. This interdisciplinary project will test the model experimentally, and vice versa, use the model to suggest key experiments. The suggested work will complement the theoretical training of the fellow with hands-on experience.'