INFLAM

Regulation of Inflammation

Coordinatore	Karlsruher Institut fuer Technologie    more  Organization address address: Kaiserstrasse 12 city: Karlsruhe postcode: 76131 contact info Titolo: Ms. Nome: Natascha Cognome: Wallburg Email: send email Telefono: +49 7247 825414 Fax: +49 7247 825403
Nazionalità Coordinatore	Germany [DE]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-IRG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-09-01   -   2014-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	Karlsruher Institut fuer Technologie  Organization address address: Kaiserstrasse 12 city: Karlsruhe postcode: 76131 contact info Titolo: Ms. Nome: Natascha Cognome: Wallburg Email: send email Telefono: +49 7247 825414 Fax: +49 7247 825403	DE (Karlsruhe)	coordinator	100˙000.00

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Despite its fundamental importance, our understanding of the immediate mechanisms directing tissue response and the complex cell-cell communications regulating its resolution remain fairly limited. Moreover, current anti-inflammatory drugs work relatively unspecific as on-off switches, thereby exhibiting considerable side effects. Studies on innate immunity have benefitted from the introduction of the transparent zebrafish larvae as an assay system. Transgenic fish expressing fluorescent proteins in leukocytes allow direct quantification of the inflammatory response in real-time in a live animal. However, the assays used to date require physical wounding of the fish preventing even a medium throughput experimental scale. We have developed a novel wounding assay without the need for mechanical wounding allowing high throughput large-scale analyses towards a comprehensive understanding of inflammation. Here, I propose the generation of an automated screening platform based on our specific ‘non-invasive’ chemical wounding followed by automated microscopy and image acquisition that will allow the in vivo screening of thousands of compounds in a matter of days with respect to their effect on leukocyte recruitment, leukocyte function, resolution of the response and general toxicity in a single assay; target profiling of small molecule ‘hits’ from by chemical proteomics and integration of acquired data into an interactive web-based repository aiming at an interconnected view of molecular networks guiding an innate immune response. This platform will allow chemical and genetic screens to the identification of new molecular pathways involved in innate immunity. At the same time, our approach will identify lead compounds for the development of novel immuno-modulatory drugs with enhanced specificity allowing fine-tuning of inflammation by targeting particular aspects of an innate immune response.'

Introduzione (Teaser)

Understanding how the process of inflammation can turn into a pathological condition requires detailed knowledge of the mechanism. European researchers optimised the zebrafish model to facilitate research into inflammation.