LIVER IVM

Imaging liver immunopathology by intravital microscopy

Coordinatore	FONDAZIONE CENTRO SAN RAFFAELE DEL MONTE TABOR    more  Organization address address: Via Olgettina 60 city: MILANO postcode: 20132 contact info Titolo: Mr. Nome: Mario Cognome: Valsecchi Email: send email Telefono: +39 02 2643 2729 Fax: +39 02 2643 2752
Nazionalità Coordinatore	Italy [IT]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-IRG
Anno di inizio	0
Periodo (anno-mese-giorno)	0000-00-00   -   0000-00-00

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	FONDAZIONE CENTRO SAN RAFFAELE DEL MONTE TABOR  Organization address address: Via Olgettina 60 city: MILANO postcode: 20132 contact info Titolo: Mr. Nome: Mario Cognome: Valsecchi Email: send email Telefono: +39 02 2643 2729 Fax: +39 02 2643 2752	IT (MILANO)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'The objective of this proposal is to take advantage of recently developed technological progresses in the field of live imaging to elucidate the pathogenesis of hepatitis B virus (HBV) infection. CD8 T cells play a major part in the development of liver disease and the resolution of HBV infection. A number of important issues pertaining to HBV pathogenesis remain unresolved and they include the way in which CD8 T cells traffic and recognize antigen within the liver and the way in which the diseased liver influences these processes. Our rationale is based on the notion that - at last - we can address these and other related issues experimentally. Single- and two-photon intravital microscopy will be carried out in un-inflamed or fibrotic/cirrhotic livers from mouse models that include transgenic mice that express all of the viral polypeptides and replicate HBV in the hepatocyte and normal mice infected with HBV-replicating, hepatotropic, fluorescent adenoviruses. The spatial and temporal constraints of naïve or effector HBV-specific CD8 T cells recognizing viral antigens and interacting intrahepatically with platelets, endothelial cells or Kupffer cells will be visualized thanks to experimental systems created specifically for this purpose. These studies will shed new light on previously unknown aspects of HBV pathogenesis, paving the road to new immunotherapeutic strategies aimed at terminating chronic HBV infection. Results from this proposal may also have far-reaching implications, not only in related liver infections (i.e. hepatitis C virus), but also in other T cell-mediated pathological conditions of the liver such as autoimmunity, graft rejection or cancer.'