RIIFERT

Regulation of innate immunity in the female reproductive tract

Coordinatore	THE UNIVERSITY OF SHEFFIELD    more  Organization address address: FIRTH COURT WESTERN BANK city: SHEFFIELD postcode: S10 2TN contact info Titolo: Ms. Nome: Joanne Cognome: Watson Email: send email Telefono: +44 114 222 4754 Fax: +44 114 222 1455
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	173˙240 €
EC contributo	173˙240 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-IIF
Funding Scheme	MC-IIF
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-01-10   -   2013-01-09

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	THE UNIVERSITY OF SHEFFIELD  Organization address address: FIRTH COURT WESTERN BANK city: SHEFFIELD postcode: S10 2TN contact info Titolo: Ms. Nome: Joanne Cognome: Watson Email: send email Telefono: +44 114 222 4754 Fax: +44 114 222 1455	UK (SHEFFIELD)	coordinator	173˙240.80

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 Obiettivo del progetto (Objective)

'Implantation failure after natural mating or in vitro fertilization may be due to either embryo or endometrial factors. Recent evidence suggests that sub-clinical infection, inflammation or malfunctions of the immune system in the female reproductive tract (FRT) are major causes of implantation failure. Toll like receptors (TLRs) have been shown to have a key role in the induction of immune and inflammatory responses in mammals. TLRs are the main family of pattern recognition receptors, they recognise pathogen-associated molecular pattern and constitute a major part of the innate immune system. We have demonstrated the presence and distribution of TLRs in the human FRT and we have also reported cyclical expression of TLRs during the menstrual cycle in the human endometrium. TLRs expression and function is also affected by sex hormones in endometrial cell lines. Finally using in vitro assays of human implantation, it is clear that activation of TLR5 leads to a reduction in binding of human trophoblast cells to endometrium. Indicative of the role that this molecule plays in mediating human implantation process. We aim to systemically investigate the effect of sex hormones on expression and function of TLR5 in Human endometrial cells. We hypothesise that alteration of sex hormones in the environment of endometrial cells will result in alterations of TLR5 expression and function. Using a TLR5 gene-reporter system, TLR5-eGFP system and microfluditics we aim to construct an in vitro model for systemic analysis of TLR5 gene expression, protein production and function in endometrial cells in the presence of different combinations and concentrations of sex hormones. Data obtained will be used to establish an in silico model to predict alterations in expression and function of TLR5 at different stages of reproductive cycle, in health and disease. Such an in silico model will have diverse applications in improving diagnosis, treatment and care for infertility patients.'