DEGRADEPLANT
Directed evolution and semi-rational engineering of plant cell wall-degrading enzymes
| Coordinatore | THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Organization address
address: The Old Schools, Trinity Lane contact info |
| Nazionalità Coordinatore | United Kingdom [UK] |
| Totale costo | 200˙049 € |
| EC contributo | 200˙049 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2010-IEF |
| Funding Scheme | MC-IEF |
| Anno di inizio | 2011 |
| Periodo (anno-mese-giorno) | 2011-06-01 - 2013-05-31 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
Organization address
address: The Old Schools, Trinity Lane contact info |
UK (CAMBRIDGE) | coordinator | 200˙049.60 |
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Obiettivo del progetto (Objective)
'This proposal focuses on the development of new techniques and strategies for the directed evolution of enzymes useful for industrial (white) biotechnology, including the development of new genetic methodologies to improve the potential of variant libraries, as well as new screening tools to broaden the range of targets for directed evolution. Specifically, the proposed project will apply these new methodologies to plant cell-wall degrading enzymes, namely cellulases and hemicellulases, which are crucial biocatalysts for the production of second-generation lignocellulose-derived biofuels. The enzymes targeted in this proposal are phylogenetically-related, share the same fold, catalyze the same chemical transformation on a large number of structurally related substrates and thus provide good starting points to switch substrate specificities by directed evolution. In order to achieve these challenging objectives, the proposed project will combine new technical advances: on the one hand methodologies to construct gene libraries and stability compensation strategies, and on the other hand new screening approaches of unprecedented throughput based on compartmentalized screening in microdroplets that are processed in microfluidic devices.'