3D HMEC-MT-TIP

Regulation of mammary epithelial morphogenesis by microtubule plus-end tracking proteins

Coordinatore	UNIVERSITEIT UTRECHT    more  Organization address address: Heidelberglaan 8 city: UTRECHT postcode: 3584 CS contact info Titolo: Ms. Nome: Astrid Cognome: Haijma Email: send email Telefono: 31302539227
Nazionalità Coordinatore	Netherlands [NL]
Totale costo	176˙685 €
EC contributo	176˙685 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-IEF
Funding Scheme	MC-IEF
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-04-01   -   2014-03-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITEIT UTRECHT  Organization address address: Heidelberglaan 8 city: UTRECHT postcode: 3584 CS contact info Titolo: Ms. Nome: Astrid Cognome: Haijma Email: send email Telefono: 31302539227	NL (UTRECHT)	coordinator	176˙685.60

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 Obiettivo del progetto (Objective)

'The mammary gland functional unit develops as a result of a complex morphogenetic process organizing polarized epithelial cells into a branched network of ducts terminating in acini. Most of human breast cancers originate from transformation of epithelial cells and induce breakdown of this structure. Studies in cells grown in two-dimensional (2D) cultures have shown that microtubules target and regulate actin network and adhesion complexes, which are also known to be essential components of three-dimensional (3D) tissue architecture. Here, I propose to address microtubule functions and behavior in a more relevant system to model physiological morphogenesis. For this purpose, I intend to investigate mechanisms underlying the regulation of microtubule dynamics and their biological impact in an in vitro reconstituted acinar model of untransformed mammary epithelial cells grown in 3D. My project aims at addressing the role of the growing family of microtubule plus-end tracking proteins (TIPs) in the control of microtubule functions during 3D mammary epithelial morphogenesis. Special focus will be given to TIP-dependent microtubule cross-talk with key structural components of acinar architecture. Imaging of microtubules in 3D mammary epithelial culture will be used to analyze microtubule behavior during epithelial-mesenchymal transition and the role of TIPs in this process.'