MM OF LE IN EME

Molecular mechanisms underlying the lineage establishment in the early mouse embryo

Coordinatore	EUROPEAN MOLECULAR BIOLOGY LABORATORY    more  Organization address address: Meyerhofstrasse 1 city: HEIDELBERG postcode: 69117 contact info Titolo: Dr. Nome: Genevieve Cognome: Reinke Email: send email Telefono: +49 6221 3878153 Fax: +49 6221 3878575
Nazionalità Coordinatore	Germany [DE]
Totale costo	170˙363 €
EC contributo	170˙363 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-IIF
Funding Scheme	MC-IIF
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-07-01   -   2013-06-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	EUROPEAN MOLECULAR BIOLOGY LABORATORY  Organization address address: Meyerhofstrasse 1 city: HEIDELBERG postcode: 69117 contact info Titolo: Dr. Nome: Genevieve Cognome: Reinke Email: send email Telefono: +49 6221 3878153 Fax: +49 6221 3878575	DE (HEIDELBERG)	coordinator	170˙363.20

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 Obiettivo del progetto (Objective)

'Early mammalian development is characterized by formation of the blastocyst, composed of the pluripotent inner cell mass surrounded by a one-cell layer of epithelial cells, the trophectoderm. Despite its importance for understanding mammalian development and for stem cell research, the mechanism of blastocyst morphogenesis and patterning has long been elusive and a subject of controversy. The ultimate goal of this project is to understand the molecular mechanism of the lineage establishment in the mouse blastocyst. To this end, functional screens will be performed to identify players essential for the lineage specification. Those gene sets specifically expressed in each cell lineage will be characterized by single-cell gene expression profiling methods. Functional screens will be performed using a subset of siRNA libraries directed against each gene set in order to identify key molecules for the blastocyst differentiation. Here, fluorescent reporter lines established in the host laboratory will serve as a unique reference for the read-out of molecular dynamics during embryonic patterning. Alternatively, among those gene sets, potential candidate molecules will be first identified by focusing to the upstream players and small RNAs in signaling cascades in the lineage specification. Their function will then be analyzed by siRNA-mediated knock-down. Thus, these findings will provide a mechanistic model underlying the lineage establishment in the early mouse embryo. Taken together, the project will provide not only an insight into the pluripotency in vivo but a basis for an interdisciplinary approach for stem cell research and regenerative medicine. It is likely that the techniques established in the project will serve as a unique tool for embryonic development research and will result in a long lasting fruitful collaboration.'