DR-NANO

Depth-Resolved Optical Nanoscopy

Coordinatore	BEN-GURION UNIVERSITY OF THE NEGEV    more  Organization address address: Office of the President - Main Campus city: BEER SHEVA postcode: 84105 contact info Titolo: Ms. Nome: Daphna Cognome: Tripto Email: send email Telefono: +972 8 6472435 Fax: +972 8 6472930
Nazionalità Coordinatore	Israel [IL]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-CIG
Funding Scheme	MC-CIG
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-09-01   -   2015-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	BEN-GURION UNIVERSITY OF THE NEGEV  Organization address address: Office of the President - Main Campus city: BEER SHEVA postcode: 84105 contact info Titolo: Ms. Nome: Daphna Cognome: Tripto Email: send email Telefono: +972 8 6472435 Fax: +972 8 6472930	IL (BEER SHEVA)	coordinator	100˙000.00

Mappa

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 Obiettivo del progetto (Objective)

'A fluorescence microscope for the noninvasive imaging of the structure and dynamics in thick 3D systems with 3D sub-diffraction-limited resolution and at extended penetration depths would have a broad spectrum of applications in both the physical and biological sciences. Conventional fluorescence microscopy techniques, such as confocal and two-photon microscopy, lack the spatial resolution required for measurements at the nanoscale, whereas state-of-the-art 3D super-resolution optical microscopy methods offer limited penetration depth (<5 microns). In the proposed project, I aim to develop a new microscope that combines molecular photoswitches, fluorescence self-interference and light-sheet microscopy to optically section a thin layer deep in thick semitransparent samples with 3D sub-diffraction-limited resolution. I will then test it for potential physical and biological applications of single molecule detection. The first specific aim is to develop a depth-resolved self-interference photoswitching nanoscope employing novel high-throughput and sub-diffraction-limit fluorescence interferometry. The second specific aim is to map with 3D nanometer resolution the motion of photoswitchable fluorescent probes deep in soft materials. This study will open up new possibilities for precise measurements of the heterogeneity and mechanical properties of the nanoenvironments of soft matter at extended depths and may ultimately assist in developing superior biomaterials and nanomedical therapeutics. The third specific aim is to in situ image with 3D nanometer resolution the muscle thick filament system in fixed Caenorhabditis elegans nematodes at both the ventral and dorsal ends (separated by ~80 microns). This demonstration, if successful, will provide unique 3D super-resolution in situ image data and may assist in developing imaging protocols for in situ nanoscopy of structural changes of the C. elegans body-wall muscle system due to sarcomeric muscle diseases.'