DOGMITE
The unique dioxygenases of phytophagous spider mites: new enzyme players in plant-herbivore interactions?
Total Cost €
0EC-Contrib. €
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Project "DOGMITE" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITEIT VAN AMSTERDAM
Organization address contact info |
| Coordinator Country | Netherlands [NL] |
| Project website | http://www.nickywybouw.org/ |
| Total cost | 165˙598 € |
| EC max contribution | 165˙598 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2014 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-09-01 to 2017-08-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITEIT VAN AMSTERDAM | NL (AMSTERDAM) | coordinator | 165˙598.00 |
Map
Project objective
Detoxification of poisonous xenobiotics in animals is typically performed by multi-gene enzyme families. Within arthropods, only insect genomes have been studied in detail where these families are well characterized. I recently uncovered a new enzyme family in the genome of a non-insect arthropod, the extremely polyphagous plant-feeding mite Tetranychus urticae and showed that this proliferated family was acquired via horizontal gene transfer from a fungal donor. The family codes for intradiol ring cleaving dioxygenases which cleave a particular set of aromatic structures, commonly found in pesticides and plant metabolites. Here, I propose to functionally characterize this exciting new gene family and to elucidate its role in xenobiotic detoxification, with a focus on plant secondary metabolites. First, to create a general picture, I will map the in situ expression of dioxygenases and time their responses to plant-derived secondary defense metabolites. Second, guided by preliminary results, I plan to study how these new herbivore enzymes counteract polyphenol oxidases, well-known plant defense enzymes that act against herbivores and target the same class of substrates. State-of-the-art plant transformation experiments will be performed in order to meticulously dissect their counterplay. Finally, by means of an unbiased multi-layered strategy, I will functionally characterize these new dioxygenases. Dioxygenases will be introduced into biological systems by functional expression in E. coli or insect cells, and by genetically transforming Drosophila. Cutting-edge differential metabolomics will identify the substrates and reaction products. By means of this project, I expect to unravel the selective advantage of this new family for phytophagous mites and open up avenues to exciting biotechnological applications which I expect to extend well beyond agriculture.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2016 |
Wim Jonckheere, Wannes Dermauw, Vladimir Zhurov, Nicky Wybouw, Jan Van den Bulcke, Carlos A. Villarroel, Robert Greenhalgh, Mike Grbić, Rob C. Schuurink, Luc Tirry, Geert Baggerman, Richard M. Clark, Merijn R. Kant, Bartel Vanholme, Gerben Menschaert, Thomas Van Leeuwen The salivary protein repertoire of the polyphagous spider mite Tetranychus urticae : a quest for effectors published pages: mcp.M116.058081, ISSN: 1535-9476, DOI: 10.1074/mcp.M116.058081 |
Molecular & Cellular Proteomics | 2019-06-14 |
| 2016 |
Nicky Wybouw, Yannick Pauchet, David G. Heckel, Thomas Van Leeuwen Horizontal gene transfer contributes to the evolution of arthropod herbivory published pages: evw119, ISSN: 1759-6653, DOI: 10.1093/gbe/evw119 |
Genome Biology and Evolution | 2019-06-14 |
| 2017 |
Gonçalo Santos-Matos, Nicky Wybouw, Nelson E. Martins, Flore Zélé, Maria Riga, Alexandre B. Leitão, John Vontas, Miodrag Grbić, Thomas Van Leeuwen, Sara Magalhães, Élio Sucena Tetranychus urticae mites do not mount an induced immune response against bacteria published pages: 20170401, ISSN: 0962-8452, DOI: 10.1098/rspb.2017.0401 |
Proceedings of the Royal Society B: Biological Sciences 284/1856 | 2019-06-14 |
| 2017 |
Nena Pavlidi, Anastasia Gioti, Nicky Wybouw, Wannes Dermauw, Michael Ben-Yosef, Boaz Yuval, Edouard Jurkevich, Anastasia Kampouraki, Thomas Van Leeuwen, John Vontas Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding published pages: 42633, ISSN: 2045-2322, DOI: 10.1038/srep42633 |
Scientific Reports 7 | 2019-06-14 |
| 2017 |
Caleb R. Schlachter, Vincent Klapper, Nicky Wybouw, Taylor Radford, Thomas Van Leeuwen, Miodrag Grbic, Maksymilian Chruszcz Structural Characterization of a Eukaryotic Cyanase from Tetranychus urticae published pages: 5453-5462, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.7b01333 |
Journal of Agricultural and Food Chemistry 65/27 | 2019-06-14 |
| 2017 |
Heike Staudacher, Bernardus Schimmel, Mart Lamers, Nicky Wybouw, Astrid Groot, Merijn Kant Independent Effects of a Herbivore’s Bacterial Symbionts on Its Performance and Induced Plant Defences published pages: 182, ISSN: 1422-0067, DOI: 10.3390/ijms18010182 |
International Journal of Molecular Sciences 18/1 | 2019-06-14 |
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