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SegregActin SIGNED

Building Distinct Actin Filament Networks in a Common Cytoplasm

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

SegregActin project word cloud

Explore the words cloud of the SegregActin project. It provides you a very rough idea of what is the project "SegregActin" about.

combined facts structurally removed certain proteins interaction model temporally abp rheological hypothesize organism interactions organize cytoskeleton protein assembled relationship variety network regulation one binding yeast geometrical extracts light depends favored conformations composition dynamical physiological defects understand reconstitutions organization near tractable players strategy fact multiple expression networks generation mechanical prepared families stabilized abps explained structures interacting combining limited original pool genetically appropriate filaments unprecedented cells diversity imposes opportunity modifications cytoplasmic interior components deformations modulate shed generate actin subunits cellular identical cerevisiae added

Project "SegregActin" data sheet

The following table provides information about the project.

Coordinator	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Total cost	1˙500˙000 €
EC max contribution	1˙500˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2014-STG
Funding Scheme	ERC-STG
Starting year	2015
Duration (year-month-day)	from 2015-09-01 to 2021-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Organization address address: RUE MICHEL ANGE 3 city: PARIS postcode: 75794 website: www.cnrs.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS)	coordinator	1˙500˙000.00
2	COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES Organization address address: RUE LEBLANC 25 city: PARIS 15 postcode: 75015 website: www.cea.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS 15)	participant	0.00

Map

Project objective

'The ability of cells to use the actin cytoskeleton for a diversity of cellular processes is due to the fact that actin filaments, although assembled from identical subunits, are organized in a wide variety of structures of appropriate geometrical, dynamical and rheological properties. Key players in this regulation are specific sets of actin binding proteins (ABPs) interacting with each actin networks, to modulate spatially and temporally their properties. With this project, I want to understand 1/ how cells can generate the formation of actin structures of appropriate ABP composition from a common pool of cytoplasmic components and 2/ the relationship between the ABP composition of an actin network, its geometrical and dynamical properties, and its response to mechanical deformations.

I will hypothesize that the generation of an actin network of appropriate ABP composition can be explained with an original model, taking into account the facts that 1/ actin filaments in cells are not all structurally identical, but adopt specific conformations that are favored and stabilized by certain families of ABPs; and 2/ the interaction of ABPs with actin depends of the geometrical organization of the filaments.

Because this project imposes to study protein-protein interactions in the presence of multiple partners, I propose to develop an unprecedented strategy combining 1/ 'bottom-up' reconstitutions, where limited sets of ABPs are added one-by-one in the system to understand their combined activities with actin; and 2/ 'top-down' reconstitutions with protein extracts prepared from a genetically-tractable organism (the yeast S. cerevisiae), where proteins can be removed one-by-one, in order to study actin network properties in near-physiological conditions.

This project will shed a new light on how cells organize their interior, and will represent a unique opportunity to understand how modifications in the expression of ABPs are associated with actin network defects. '

Publications

List of publications.
year	authors and title	journal	last update
2017	Gergana Gateva, Elena Kremneva, Theresia Reindl, Tommi Kotila, Konstantin Kogan, LaurÃ¨ne Gressin, Peter W. Gunning, Dietmar J. Manstein, AlphÃ©e Michelot, Pekka Lappalainen Tropomyosin Isoforms Specify Functionally Distinct Actin Filament Populations InÂ Vitro published pages: 705-713, ISSN: 0960-9822, DOI: 10.1016/j.cub.2017.01.018	Current Biology 27/5	2020-02-12
2019	Adrien Antkowiak, Audrey Guillotin, Micaela Boiero Sanders, Jessica Colombo, Renaud Vincentelli, AlphÃ©e Michelot Sizes of actin networks sharing a common environment are determined by the relative rates of assembly published pages: e3000317, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.3000317	PLOS Biology 17/6	2020-02-12
2019	Jessica Planade, Reda Belbahri, Micaela Boiero Sanders, Audrey Guillotin, Olivia du Roure, AlphÃ©e Michelot, Julien Heuvingh Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency published pages: e3000500, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.3000500	PLOS Biology 17/10	2020-02-12

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The information about "SEGREGACTIN" are provided by the European Opendata Portal: CORDIS opendata.