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Cells-in-drops

High throughput screening of single-cells using droplet microfluidics

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

Cells-in-drops project word cloud

Explore the words cloud of the Cells-in-drops project. It provides you a very rough idea of what is the project "Cells-in-drops" about.

chain loosing biomolecules advantage profile branches biological sciences primary hard populations functional scalability platform conventional dna quantitative microfluidics biology mrna igg technologies single outlined microfluidic binding molecule biotechnology proteins heterogeneous throughput date analytical regulatory cloning direct genotype original genes antibodies linkage beads cell therapeutic ultra demand encoding heavy light cells industrial barcoded compartmentalization gene performance phenotype tackling technological biomedicine capture pairing phenotyping perform immortalization secreted resolution droplet establishment antibody primers monoclonal droplets purify fulfill selectively techniques sequencing screening sequence

Project "Cells-in-drops" data sheet

The following table provides information about the project.

Coordinator	VILNIAUS UNIVERSITETAS Organization address address: UNIVERSITETO G. 3 city: VILNIUS postcode: 1513 website: http://www.vu.lt contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Lithuania [LT]
Project website	http://www.bti.vu.lt/en/departments/sector-of-microtechnologies
Total cost	130˙779 €
EC max contribution	130˙779 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-EF-RI
Starting year	2016
Duration (year-month-day)	from 2016-03-21 to 2018-03-20

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	VILNIAUS UNIVERSITETAS VILNIAUS UNIVERSITETAS Organization address address: UNIVERSITETO G. 3 city: VILNIUS postcode: 1513 website: http://www.vu.lt contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	LT (VILNIUS)	coordinator	130˙779.00

Map

Project objective

Tackling heterogeneous cell populations at single-cell resolution is becoming increasingly important in different branches of biology and biomedicine. Many useful techniques have been developed to profile and even selectively purify single-cells, however, the demand for techniques with better analytical performance and improved high-throughput capabilities, remains very high. Droplet microfluidics can fulfill this demand by bringing higher throughput, scalability and single molecule resolution that are hard to achieve with conventional technologies. In this project, a droplet microfluidics platform will be developed and applied for ultra-high-throughput single-cell screening and sequencing. The project will be focused on B-cells that produce therapeutic antibodies or biomolecules of industrial interest. Cell compartmentalization into microfluidic droplets together with capture beads and barcoded DNA primers will enable a direct establishment of the linkage between the genotype (genes or mRNA) and phenotype (binding, regulatory or activity of secreted proteins). The proposed work will allow the quantitative high-throughput antibody phenotyping without loosing the original heavy-light chain pairing, a significant advantage over other technologies. Like no other system available to-date this the technological approach outlined in this proposal will provide a unique way to identify the primary sequence of heavy and light IgG genes encoding functional monoclonal antibodies directly from single-cells, without a need to perform gene cloning or cell immortalization. The results of this work are likely to bring a significant impact not only in applied biological sciences but also in biotechnology and biomedicine.

Publications

List of publications.
year	authors and title	journal	last update
2016	Rapolas Zilionis, Juozas Nainys, Adrian Veres, Virginia Savova, David Zemmour, Allon M Klein, Linas Mazutis Single-cell barcoding and sequencing using droplet microfluidics published pages: 44-73, ISSN: 1754-2189, DOI: 10.1038/nprot.2016.154	Nature Protocols 12/1	2019-07-26
2017	Greta Zubaite, Karolis Simutis, Robertas Galinis, Valdemaras Milkus, Vaidotas Kiseliovas, Linas Mazutis Droplet Microfluidics Approach for Single-DNA Molecule Amplification and Condensation into DNA-Magnesium-Pyrophosphate Particles published pages: 62, ISSN: 2072-666X, DOI: 10.3390/mi8020062	Micromachines 8/2	2019-07-26

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