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TOC-maker SIGNED
The assembly and structure of the chloroplast protein import machinery in plants
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0TOC-maker project word cloud
Explore the words cloud of the TOC-maker project. It provides you a very rough idea of what is the project "TOC-maker" about.
affinity
experiments
configurations
toc
interacting
tightly
biochemical
etiolation
reported
translocons
stalled
enhanced
compartment
predicted
nature
ribosomes
epitope
encoded
chloroplasts
equipped
composition
protein
machinery
complexes
cryo
analysing
assembly
biogenesis
assist
energy
sequence
rapid
tagged
synthesis
purpose
events
components
structural
pulse
import
molecular
chloroplast
cells
assembled
housekeeping
photosynthetic
apparatus
purification
homeostasis
techniques
nucleus
specialized
microscopy
convert
cell
chase
organization
purified
chemical
mature
membranes
outer
abundant
photosynthesis
respectively
solar
transgenic
tic
electron
difficult
polypeptides
multiprotein
plant
majorly
proteins
resolution
arabidopsis
plants
nascent
model
gap
enriched
mechanisms
elucidated
expressing
thaliana
proper
integration
dynamic
envelope
inner
transiently
de
coupled
Project "TOC-maker" data sheet
The following table provides information about the project.
| Coordinator |
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Total cost | 224˙933 € |
| EC max contribution | 224˙933 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2020 |
| Duration (year-month-day) | from 2020-06-01 to 2022-05-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD | UK (OXFORD) | coordinator | 224˙933.00 |
Map
Project objective
Plants convert solar energy into chemical energy by the process called photosynthesis in a specialized compartment of the cell known as chloroplasts. Chloroplasts are majorly enriched with nucleus-encoded proteins and to import them, chloroplast outer and inner envelope membranes are equipped with apparatus called the TOC and TIC translocons, respectively. For the TOC apparatus, there are two major configurations, TOC-P and TOC-H, reported so far, which import highly abundant, Photosynthetic and Housekeeping pre-proteins, respectively. TOC-P and TOC-H are multiprotein complexes which must be specifically assembled for proper development and homeostasis of the chloroplast. Due to the dynamic nature of the translocons, component synthesis and assembly must be rapid and tightly coupled, making the process difficult to investigate. Thus, understanding the mechanisms of the assembly process is both challenging and exciting. Biogenesis of TOC complexes is rapidly enhanced during chloroplast development or de-etiolation, and I will exploit this process to investigate the assembly of different TOC configurations, using the model plant Arabidopsis thaliana. For this purpose, transgenic plants expressing epitope-tagged TOC components and cells expressing nascent polypeptides of TOC components with stalled ribosomes will be generated. Proteins transiently interacting with new TOC components, which are predicted to assist integration and assembly of the TOC complex, will be studied by using affinity purification, pulse-chase experiments, and other biochemical techniques, and thus a sequence of assembly events will be elucidated. Although the molecular composition of the TOC protein import machinery has been well studied, the detailed structural organization of TOC complexes has not yet been elucidated. I will address this knowledge gap by analysing affinity-purified TOC complexes from mature chloroplasts at high resolution by cryo-electron microscopy.
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The information about "TOC-MAKER" are provided by the European Opendata Portal: CORDIS opendata.
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