MAZPROTEC

Two-Step-Strategy towards an Orthogonal Bio-System

Coordinatore	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH    more  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Sven Cognome: Panke Email: send email Telefono: +4161387 3209
Nazionalità Coordinatore	Switzerland [CH]
Totale costo	184˙709 €
EC contributo	184˙709 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-IEF
Funding Scheme	MC-IEF
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-03-01   -   2014-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH  Organization address address: Raemistrasse 101 city: ZUERICH postcode: 8092 contact info Titolo: Prof. Nome: Sven Cognome: Panke Email: send email Telefono: +4161387 3209	CH (ZUERICH)	coordinator	184˙709.40

Mappa

 Word cloud

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 Obiettivo del progetto (Objective)

'The integration of orthogonality is a crucial research strategy in the rational design of biological systems for novel applications. In order to produce such highly predictable orthogonal systems, we propose to employ cell-free systems of highly engineered composition generated from living cells, which are complex enough to reproduce the major synthetic capabilities of living cells - such as the synthesis of natural and artificial saccharides - but are simplified enough to come close to truly engineerable systems. We propose a two-step-strategy: In a first step, the protein synthesis of a growing bacterial cell will be channeled solely to a limited set of system components with the help of the RNA-interferase MazF. In a second step, the cells will be homogenized and the resulting cell-free extract, already enriched in the required protein components, will be subjected to selective hydrolysis of predefined proteins, which otherwise would connect the designed system to the remaining protein background, which would make the performance of the complex system unpredictable. As a proof-of-concept, we propose to implement a preparative 12-step synthesis from cheap glucose and N-acetyl-glucosamine to a valuable antiviral-precursor, N-acetyl-neuraminic acid.'

Introduzione (Teaser)

The synthesis of chemical compounds for industry now includes application of biological catalysts (BCs). BCs, or enzymes, play an important role due to their unparalleled selectivity and ability to carry out multiple reactions concomitantly in one reactor.