Coordinatore | UNIVERSITY OF LEEDS
Organization address
address: WOODHOUSE LANE contact info |
Nazionalità Coordinatore | United Kingdom [UK] |
Totale costo | 270˙145 € |
EC contributo | 270˙145 € |
Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
Code Call | FP7-PEOPLE-2011-IIF |
Funding Scheme | MC-IIF |
Anno di inizio | 2012 |
Periodo (anno-mese-giorno) | 2012-06-01 - 2014-05-31 |
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UNIVERSITY OF LEEDS
Organization address
address: WOODHOUSE LANE contact info |
UK (LEEDS) | coordinator | 270˙145.80 |
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'The lifespan of crop plants is crucial for agricultural production. In particular, the duration of leaf photosynthesis and the stability of chloroplast proteins are important determinants of grain yields. Environmental stresses such as chilling and drought impair the functions of chloroplast proteins and inhibit photosynthesis leading to premature senescence and yield losses. Despite its paramount importance in crop productivity and quality, lifespan control has remains poorly characterised and understood, particularly in grain legume crops. This project focuses on lifespan controls in soybean leaves (as the source of assimilated carbon metabolites) and nodules (as the source of assimilated nitrogen metabolites). Crucially, this project will determine how the lifespan of these organs is altered in transgenic plants by ectopic expression of cysteine and serine protease inhibitors. The transgenic soybean lines required for these studies have already been generated in order to test and establish proof of the concept that manipulation of protease inhibitors can effectively extend organ lifespan especially under stress conditions. The transgenic lines express either the rice cystatin, (OC-I), a maize serpin or a Bowman Birk (BBI) type inhibitor. The effects of OC-I expression in soybean chloroplasts will be compared with cytosolic expression on key parameters such as photosynthesis, growth and development under optimal, drought and chilling stress conditions. In addition, the effects of ectopic expression of the BBI inhibitor and the serpin on leaf and nodule development will be studied, together with effects of targeted OC-I expression in nodules. Combining the extensive knowledge and experience of the fellow in protease inhibitors with the expertise of the host lab in stress biology, key scientific questions will be addressed while assisting the fellow to further his career in entrepreneurial consultancy, knowledge brokering and technology transfer.'
Researchers are identifying genes and physical traits that affect the performance and lifespan of soybean crops during drought and nitrogen stress.
Soybean is the fourth most important crop worldwide. However, crop yield is severely hampered by environmental conditions, which ultimately has a detrimental effect on the food security of millions of people.
To address this problem, the EU-funded 'Extending soybean lifespan' (SOYLIFE) project aimed to enhance the lifespan and yield of soybean. By studying gene function and whole-plant physiology, the project hoped to provide useful insights into stresses like drought and low nitrogen.
Researchers successfully expressed a rice gene in soybean through genetic engineering techniques. This naturally occurring protein, oryzacystatin, interacts with soybean growth hormones. The result: plants have higher protein content, have higher photosynthetic rates and are more stress tolerant.
A myriad of other genes were also found to be active in the roots during low-nitrogen conditions. These all have the potential to play an important role in superior soybean varieties and were kept in a database at Leeds University (United Kingdom) for further study.
Through its proof-of-concept, project outcomes have the potential to underpin the production of a new generation of high-performance plants. Furthermore, the knowledge and experienced gleaned from this project will be transferred to research groups in Africa. Researchers will have the opportunity to design and increase crop production for increased food security and economic value where it is most needed.