VISUALDENDRITE

In vitro and in vivo examination of the spatial and temporal distribution of synaptic inputs and synaptic integration in layer 2/3 visual cortical neurons

Coordinatore	INSTITUT PASTEUR    more  Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 contact info Titolo: Dr. Nome: Marie-Laure Cognome: Rosso Email: send email Telefono: +33 1 44 38 95 26 Fax: +33 1 40 61 39 40
Nazionalità Coordinatore	France [FR]
Totale costo	193˙594 €
EC contributo	193˙594 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-IEF
Funding Scheme	MC-IEF
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-05-22   -   2015-05-21

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	INSTITUT PASTEUR  Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 contact info Titolo: Dr. Nome: Marie-Laure Cognome: Rosso Email: send email Telefono: +33 1 44 38 95 26 Fax: +33 1 40 61 39 40	FR (PARIS CEDEX 15)	coordinator	193˙594.80

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 Obiettivo del progetto (Objective)

'A particularity of neurons in sensory cortex is to be tuned to specific stimulation features ; for example, in the primary visual cortex, some neurons are selective for the orientation of the stimulus. The large dendritic trees of pyramidal cortical neurons extend over hundreds of microns, allowing neurons to receive thousands of synaptic inputs. Neurons integrate and compute these inputs to produce an action potential output that will excite target cells. The dendritic tree likely serves as a substrate for these computations, through the interaction between synaptic inputs, the tree topology, and the distributions of intrinsic channels in dendrites. Elucidating the mechanisms by which dendrites implement these computations in response to functional stimuli is crucial to the understanding of how stimulus-specificity is performed in sensory neurons. Thin dendrites are not accessible to patch-clamp, but two-photon microscopy allows the measurement of [Ca2] and voltage fluctuations using fluorescent reporters. We will investigate these questions in rat visual cortical neurons in vitro (in response to photoactivation of group of synapses), and in vivo (in response to visual stimulation), by measuring optically the dynamics of voltage and Ca2 in single dendrites, in combination with somatic electrophysiological recordings and mathematical modeling of dendritic computations.'