ECT-SPEC-DIFF-MORPH

"From Gene Regulatory Networks to Specification, Differentiation and Morphogenesis in the sea urchin embryo"

Coordinatore	UNIVERSITY OF HAIFA    more  Organization address address: "Mount Carmel, Abba Khoushi Blvd." city: HAIFA postcode: 31905 contact info Titolo: Mrs. Nome: Iris Cognome: Levi Email: send email Telefono: +972 4 8288485
Nazionalità Coordinatore	Israel [IL]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-CIG
Funding Scheme	MC-CIG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-09-01   -   2016-12-07

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITY OF HAIFA  Organization address address: "Mount Carmel, Abba Khoushi Blvd." city: HAIFA postcode: 31905 contact info Titolo: Mrs. Nome: Iris Cognome: Levi Email: send email Telefono: +972 4 8288485	IL (HAIFA)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'A fundamental challenge in developmental biology is to understand how regulatory networks transform regulatory information into cell fate specification and differentiation. Addressing this challenge requires a comprehensive analysis of the regulatory connections between the transcription factors present in a cell and their regulatory and structural targets through developmental time. The sea urchin aboral ectoderm provides an excellent system to dissect these connections due to its accessibility to quantitative expression studies and the multiple developmental programs executed in this territory. One of these programs drives the differentiation of the entire aboral ectoderm into squamous epithelium and represses neural fate, while other programs define regulatory sub-domains within the aboral ectoderm important to normal morphogenesis of other embryonic territories. These programs depend strongly on the BMP pathway, a highly conserved regulator of ecotdermal patterning. Here we propose to identify the downstream targets of the aboral ectoderm regulatory network and to construct a mechanistic model of the aboral ectoderm specification and differentiation. We will conduct a genome-wide identification of (1) cis-regulatory elements that the aboral ectoderm transcription factors bind to (2) genes that respond to perturbation of the aboral ectoderm transcription factors. The function of identified cis-regulatory elements will be tested using a new high-throughput technology. The expression of key target genes will be perturbed and their effect on the aboral ectoderm morphology and embryogenesis will be studied. Based on this analysis we will construct a mathematical model that simulates the dynamic function of the aboral ectoderm regulatory network. Thus, this project will provide unprecedented understanding of the dynamic regulation of an entire developmental process, from regulatory state establishment to the acquisition of cellular and morphological properties.'