HOTDROPS

Ultra High-throughput platform for the selection of thermostable proteins by thermophilic in vitro transcription-translation and microfluidics

Coordinatore	UNIVERSIDAD AUTONOMA DE MADRID    more  Organization address address: CALLE EINSTEIN, CIUDAD UNIV CANTOBLANCO RECTORADO 3 city: MADRID postcode: 28049 contact info Titolo: Ms. Nome: Mª Carmen Cognome: Puerta Email: send email Telefono: 34914978663 Fax: 34914975269
Nazionalità Coordinatore	Spain [ES]
Totale costo	1˙973˙797 €
EC contributo	1˙973˙797 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-IAPP
Funding Scheme	MC-IAPP
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-06-01   -   2017-05-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSIDAD AUTONOMA DE MADRID  Organization address address: CALLE EINSTEIN, CIUDAD UNIV CANTOBLANCO RECTORADO 3 city: MADRID postcode: 28049 contact info Titolo: Ms. Nome: Mª Carmen Cognome: Puerta Email: send email Telefono: 34914978663 Fax: 34914975269	ES (MADRID)	coordinator	372˙262.28
2	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE  Organization address address: The Old Schools, Trinity Lane city: CAMBRIDGE postcode: CB2 1TN contact info Titolo: Ms. Nome: Renata Cognome: Schaeffer Email: send email Telefono: +44 1223 333543 Fax: +44 1223 332988	UK (CAMBRIDGE)	participant	437˙143.53
3	DROP-TECH LTD  Organization address address: "Canterbury Street, Canterbury Court 91" city: Cambridge postcode: CB4 3QU contact info Titolo: Dr. Nome: Liisa Cognome: Van Vliet Email: send email Telefono: +4478012 34598	UK (Cambridge)	participant	330˙341.44
4	NOVOZYMES A/S  Organization address address: Krogshoejvej 36 city: BAGSVAERD postcode: 2880 contact info Titolo: Dr. Nome: Ali Cognome: Fallah Araghi Email: send email Telefono: +45 44461670	DK (BAGSVAERD)	participant	287˙301.70
5	GALCHIMIA SA  Organization address address: LUGAR DE CEBREIRO SN city: O PINO A CORUNA postcode: 15823 contact info Titolo: Ms. Nome: María José Cognome: Varela Email: send email Telefono: +34 981 814 506 Fax: +34 981 814 507	ES (O PINO A CORUNA)	participant	172˙542.72
6	UNIVERSIDADE DA CORUNA  Organization address address: CALLE DE LA MAESTRANZA 9 city: LA CORUNA postcode: 15001 contact info Titolo: Dr. Nome: María Isabel Cognome: González Siso Email: send email Telefono: 34981167000 Fax: 34981167065	ES (LA CORUNA)	participant	143˙949.48
7	UNIVERSIDAD DE VIGO  Organization address address: LG CAMPUS LAGOAS MARCOSENDE city: VIGO PONTEVEDRA postcode: 36310 contact info Titolo: Mr. Nome: Anxo Cognome: Moreira Email: send email Telefono: 34986813753 Fax: +34986813 919	ES (VIGO PONTEVEDRA)	participant	117˙397.46
8	BIOCHEMIZE SL  Organization address address: CALLE DE LA INNOVACIO 2 city: TERRASSA postcode: 8225 contact info Titolo: Dr. Nome: Jaume Cognome: Mir Email: send email Telefono: 34686441120 Fax: 34937891906	ES (TERRASSA)	participant	112˙859.12

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 Obiettivo del progetto (Objective)

'This initiative connects four companies and four academic groups in pursuit of a central biotechnological challenge: to identify and improve enzymes for application in industrial biocatalysis. The technology interchange is centered around key technologies to generate thermostabilized proteins. We will develop, share and spread the following approaches (i) folding interference assays with a thermostable fluorescent protein reporter, (ii) high throughput assays in microfluidic droplets that allow screening of libraries on the order of 10e8, (iii) in vitro expression in microdroplets using expression extracts from themophiles, and (iv) approaches to generate enzyme libraries from metagenomic sources and by randomization of existing proteins. Suitable assays will be established for a list of target enzymes of interest to the industrial partners (most prominently hydrolases – such as esterases, cellulases and proteases), ensuring wide applicability and industrial relevance. Improved mutants will be characterized biochemically and scale-up and optimization for protein production by fermentation techniques will be attempted. An experienced partner who will enable others by workshop training, interchange of technical and academic personnel and joint projects represents each of these approaches. The objective of this application is to make the considerable technical skill involved in molecular biology, enzymology and screening technologies available to a wider circle of users, ranging from academic labs to very recent spin-off companies and the world’s largest provider of industrial enzymes.'