AXOTRANSMAP

Assembling a functional map of axonal signalling endosomes

Coordinatore	UNIVERSITY COLLEGE LONDON    more  Organization address address: GOWER STREET city: LONDON postcode: WC1E 6BT contact info Titolo: Ms. Nome: Kolasinska Cognome: Kamila Email: send email Telefono: 442031000000 Fax: 442078000000
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	221˙606 €
EC contributo	221˙606 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-IEF
Funding Scheme	MC-IEF
Anno di inizio	2013
Periodo (anno-mese-giorno)	2013-11-01   -   2015-10-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITY COLLEGE LONDON  Organization address address: GOWER STREET city: LONDON postcode: WC1E 6BT contact info Titolo: Ms. Nome: Kolasinska Cognome: Kamila Email: send email Telefono: 442031000000 Fax: 442078000000	UK (LONDON)	coordinator	221˙606.40

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 Obiettivo del progetto (Objective)

'The polarisation of neurons in somatodendritic and axonal compartments allows signal propagation over long distances. Despite the important role played by electrical signals, fast axonal transport is crucial for long-range communication between the soma and distal synapses, which is carried out by vesicular transport mechanisms. Neurotrophins and their receptors are internalised at axon terminals by clathrin-mediated endocytosis and conveyed by signalling endosomes to the soma, where they activate transcriptional responses regulating neuronal homeostasis and survival. However, little is known about the identity and the dynamics of the organelles involved. The aim of my project is to build a functional and physical map of axonal retrograde carriers in specific neuronal types, which are the target of important human diseases. For this purpose, I will exploit a new developed affinity purification strategy using the binding fragment of tetanus neurotoxin (HC) conjugated to monocrystalline iron oxide nanoparticles (MIONs). At different time points after internalisation, retrograde carriers will be magnetically purified and submitted to mass-spectrometry analysis. The kinetics of endosomal maturation will be assessed using quantitative label-free SILAC protocols. These results will uncover new components of signalling endosomes and identify dysfunctions of this pathway that are at the basis of motor and sensory neuropathies.'