MICRO-RIP

Functional analysis of uncultivated microbes using radioisotope probing

Coordinatore	JYVASKYLAN YLIOPISTO    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	Finland [FI]
Totale costo	1˙997˙912 €
EC contributo	1˙997˙912 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2013-CoG
Funding Scheme	ERC-CG
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-03-01   -   2019-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	JYVASKYLAN YLIOPISTO  Organization address address: SEMINAARINKATU 15 city: JYVASKYLA postcode: 40100 contact info Titolo: Dr. Nome: Marja Cognome: Tiirola Email: send email Telefono: +358 408053932 Fax: +358 14617239	FI (JYVASKYLA)	hostInstitution	1˙997˙912.80
2	JYVASKYLAN YLIOPISTO  Organization address address: SEMINAARINKATU 15 city: JYVASKYLA postcode: 40100 contact info Titolo: Prof. Nome: Mikko Cognome: Mönkkönen Email: send email Telefono: 358504000000	FI (JYVASKYLA)	hostInstitution	1˙997˙912.80

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 Obiettivo del progetto (Objective)

'Environmental microbiology calls for more advanced research methods for resolving microbial functions and regulation in environmental samples. The proposed project introduces an unconventional technology, radioisotope probing (RIP). This approach is based on the invention of radioactivity measurement via pH analysis. When the analysis of radioactivity is now combined with semiconductor sequencing, the technology offers two-dimensional analysis of millions of molecules on a sequencing chip. Hybridization of experimentally labeled RNA and radioactivity measurement would provide a second dimension for the sequencing analysis, facilitating various new applications in environmental microbiology, as well as potential applications for different needs of biochemistry and medical research. The project aims to develop and test three novel RIP applications to analyse functional diversity, transcriptional regulation and mRNA processing in microbial communities, especially focusing on prokaryotic species. The applications are utilized for studying regulation of microbial decomposition, which is the key question, when predicting the effects of climate change. It is hypothesized that more frequent flood, droughts and redox fluctuations can prime the biodegradation of otherwise stable boreal carbon pools. Functional diversity of microbes utilizing model and complex substrates is studied in terrestrial and aquatic environments using time-series samplings and labeling experiments. Prevailing mechanisms in the cellular regulation in microbial communities are investigated using community-level methylation and regulatory RNA patterns. The effect of external stressor (toxicants and changing oxygen regimes) on these patterns is analyzed using sequencing and RIP to reveal the mechanisms regulating the processes beyond mere community composition.'