COD LARVAL SURVIVAL

Elucidating the role of microbial communities in cod larval death and survival

 Coordinatore NORGES TEKNISK-NATURVITENSKAPELIGEUNIVERSITET NTNU 

 Organization address address: HOGSKOLERINGEN 1
city: TRONDHEIM
postcode: 7491

contact info
Titolo: Prof.
Nome: Olav
Cognome: Vadstein
Email: send email
Telefono: +47 73594058
Fax: +47 73591283

 Nazionalità Coordinatore Norway [NO]
 Totale costo 322˙614 €
 EC contributo 322˙614 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2013-IIF
 Funding Scheme MC-IIF
 Anno di inizio 2014
 Periodo (anno-mese-giorno) 2014-04-01   -   2016-03-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    NORGES TEKNISK-NATURVITENSKAPELIGEUNIVERSITET NTNU

 Organization address address: HOGSKOLERINGEN 1
city: TRONDHEIM
postcode: 7491

contact info
Titolo: Prof.
Nome: Olav
Cognome: Vadstein
Email: send email
Telefono: +47 73594058
Fax: +47 73591283

NO (TRONDHEIM) coordinator 322˙614.80

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microscopy    ntnu    larvae    berkeley       university    commercial    fish    gastrointestinal    electron    microbial    norwegian    aquaculture    bacteria    significant    imaging    cod    designed    tract    infection    survival   

 Obiettivo del progetto (Objective)

'This proposal contributes to solve a significant problem in commercial aquaculture: namely the high mortality of fish larvae caused by microbial infection. To enhance the survival of cod larvae I will gain in-depth insight into the microbiome of the cod larvae gastrointestinal tract by using novel and advanced correlative three-dimensional cryogenic-transmission electron microscopy and fluorescence in situ hybridization, and large-volume high-resolution focused ion beam scanning electron microscopy imaging approaches that were developed at the University of California, Berkeley and the Lawrence Berkeley National Laboratory in the U.S. I will adapt and apply these state-of-the-art imaging techniques to a research effort at the Norwegian University of Science and Technology (NTNU) designed to replace trial-and-error management of bacteria in larval cod production with an evidence-based gnotobiotic system that has been developed at NTNU. This system reduces the inherent complexity of microbial control in the wild with a carefully designed protocol beginning with bacteria-free cod larvae, and introducing bacteria stepwise as the larvae develop. Specifically I will address the following three questions: (1) Do different microbial conditions have an effect on the survival, growth and development of the fish larvae? (2) Do probionts and pathogens colonize the same region of the gastrointestinal tract? (3) What happens to the indigenous intestinal microbiota and the host during infection? By adapting these novel technologies in the context of commercial/industrial aquaculture I will not only create long-term collaborations and mutual co-operation between Europe and the U.S., but also significantly contribute to the wellbeing of the commercial aquaculture industry, which is of significant importance to both the Norwegian as well as the European economy. Thus, my proposed efforts will contribute to both European scientific excellence and European commercial competitiveness.'

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