NANOFLUIDIC-SMFD
Nanofluidic devices for high-throughput single-molecule-fluorescence detection
| Coordinatore | WAGENINGEN UNIVERSITY
Organization address
address: DROEVENDAALSESTEEG 4 contact info |
| Nazionalità Coordinatore | Netherlands [NL] |
| Totale costo | 100˙000 € |
| EC contributo | 100˙000 € |
| Programma | FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) |
| Code Call | FP7-PEOPLE-2013-CIG |
| Funding Scheme | MC-CIG |
| Anno di inizio | 2014 |
| Periodo (anno-mese-giorno) | 2014-03-01 - 2018-02-28 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
WAGENINGEN UNIVERSITY
Organization address
address: DROEVENDAALSESTEEG 4 contact info |
NL (WAGENINGEN) | coordinator | 100˙000.00 |
Mappa
Word cloud
Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.
Obiettivo del progetto (Objective)
'Currently, the two prominent schemes for single-molecule fluorescence detection (SMFD), confocal microscopy and camera-based total-internal-reflection or wide-field microscopy, are ultimately limited in their ability to combine the detection of many molecules with obtaining data at sufficiently high time resolution, necessary for resolving fast dynamics with single-molecule fluorescence resonance energy transfer (smFRET). In particular, monitoring enzymatic reactions using smFRET is extremely challenging and remains to a large extent unexplored.
Here, I propose a novel nanofluidic device to overcome these limitations by using nanochannels, which provide a well-defined flow path for a fluorescent species through the excitation/detection focus of a conventional wide-field microscope. Using an array of nanochannels offers several advantages: First, the geometrical confinement enables long observation times of non-immobilized molecules. Second, the residence time of molecules in the channels is easily controlled by the flow velocity set by the syringe pump. Third, faster flow rates together with using a CCD camera in ‘streaking mode’ enable a sub-millisecond time resolution. Fourth, a high-throughput detection is achieved by using a parallel array of channels. Fifth, enzymatic reactions can be directly triggered by mixing necessary components on-the-fly using an additional inlet.
The described device will pave the way for high-throughput single-molecule detection, which will greatly expand the possibilities for researchers to apply single-molecule methods in the area of Life Sciences.'