INFEMEC

Nanomechanics of proteins involved in viral and bacterial infections

Coordinatore	Asociacion - Centro de Investigacion Cooperativa en Nanociencias - CIC NANOGUNE    more  Organization address address: Tolosa Hiribidea 76 city: San Sebastian postcode: 20018 contact info Titolo: Mr. Nome: Miguel Cognome: Odriozola Email: send email Telefono: +34 943 57 4000 Fax: +34 943 57 4001
Nazionalità Coordinatore	Spain [ES]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-CIG
Funding Scheme	MC-CIG
Anno di inizio	2015
Periodo (anno-mese-giorno)	2015-03-01   -   2019-02-28

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	Asociacion - Centro de Investigacion Cooperativa en Nanociencias - CIC NANOGUNE  Organization address address: Tolosa Hiribidea 76 city: San Sebastian postcode: 20018 contact info Titolo: Mr. Nome: Miguel Cognome: Odriozola Email: send email Telefono: +34 943 57 4000 Fax: +34 943 57 4001	ES (San Sebastian)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'Viruses and bacteria utilize proteins to attach and infect cells and tissues. Viruses have envoloped proteins that especifically recongnize receptors in the surface of the target cells. HIV-1 recognices receptor CD4 in the surface of T cell throghout its envolope glycoprotein gp120. In the case of bacteria, they attach to tissues using long filament called pilus. Bacterial pilus type 1 is composed of several protein subunit arranged in chain, FimA-FimG-FimG-FimH. The more external domain, FimH, is the adhesin binding domain that establishes the mechanical anchoring to tissues. Both, bacterial and viral proteins withstand mechanical forces than can go from few piconewtons to hundreds. However, very little is known about how force modify the structure and features of these proteins and ultimately how its affects infection. In this project we aim to investigate the effect of mechanical forces in the anchoring proteins and its role during attachment. We will concentrate in viral receptors CD4 and bacterial fimbriae proteins (Fim). We will use a novel atomic force spectometer that allows us to apply calibrated forces to a single protein molecule. This technique allows also monitoring chemical reaction under force such as the reduction of disulfide bonds or the binding of peptides and antibodies. These processes are known to be implicated in the infection of viruses and bacteria and they may have a mechanical origin. We will use bioinformatics and high-throughout screening techniques to identify molecules that alter the nanomecanichs of these anchoring proteins and that can potentially be used to prevent infections.'