SAMDBC

Simple Atto Molar Detection and Nanoscale Kinetics of Biomolecules

Coordinatore	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD    more  Organization address address: University Offices, Wellington Square city: OXFORD postcode: OX1 2JD contact info Titolo: Ms. Nome: Gill Cognome: Wells Email: send email Telefono: +44 1865 289800 Fax: +44 1865 289801
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	231˙283 €
EC contributo	231˙283 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-IEF
Funding Scheme	MC-IEF
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-07-02   -   2016-07-01

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD  Organization address address: University Offices, Wellington Square city: OXFORD postcode: OX1 2JD contact info Titolo: Ms. Nome: Gill Cognome: Wells Email: send email Telefono: +44 1865 289800 Fax: +44 1865 289801	UK (OXFORD)	coordinator	231˙283.20

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Single Nano-Particles undergoing Brownian motion in solution colliding at an inert ultramicroelectrode, can serve as nano-electrodes during the time of contact. This provides an entirely new approach to nanoelectrochemistry. Hitherto, this new technique has been used for understanding simple redox reactions. We propose to experimentally study the electron transfer kinetics of single redox active proteins under steady state diffusion conditions, in this elegant configuration. In this way realistic mimicking of electron transfer based natural events will be achieved. In addition, the fundamentals of biological electron transfer will be revisited and re-evaluated. In parallel to this, we plan to use the natural redox activity of selected amino acids within a protein to establish a simple type of biosensor, with approximately attomolar detection sensitivity for label free proteins. Recently, the ability to observe electrocatalytic activity of single NP’s (due to collision with an electrode) was established. In the same way, we plan to use proteins as nanoparticles for biosensing applications. By immobilizing specific aptamers on an inert substrate, the absorption of label free proteins on top of the aptamers will be tracked. Chronoamperometric profiles measured on ultramicroelectrode under potential control should allow detection of attomolar concentrations.'