BACTEROMETRICS

Bioremediation of toxic metals and radionuclides using naturally evolved bacteria capable of intra-cellular reduction without oxidative stress

Coordinatore	COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES    more  Organization address address: RUE LEBLANC 25 city: PARIS 15 postcode: 75015 contact info Titolo: Dr. Nome: Catherine Cognome: Berthomieu Email: send email Telefono: 33442254384 Fax: 33442252625
Nazionalità Coordinatore	France [FR]
Totale costo	239˙736 €
EC contributo	239˙736 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-4-1-IOF
Funding Scheme	MC-IOF
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-05-01   -   2011-04-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES  Organization address address: RUE LEBLANC 25 city: PARIS 15 postcode: 75015 contact info Titolo: Dr. Nome: Catherine Cognome: Berthomieu Email: send email Telefono: 33442254384 Fax: 33442252625	FR (PARIS 15)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'Most toxic metals and radionuclides can be naturally rendered insoluble-immobile by bacterial reduction, which may be exploited for bioremediation purposes at waste repository sites. Some bacteria (e.g. iron or sulphate respirers) are receiving intense attention as their envelope-located electron transport chain can carry out such reductions. However, this process is subject to inhibition by nitrate and oxygen, usually present at waste sites. It also involves 1 e- transfer reactions generating reactive oxygen species which poison the cells and hamper remediation, and the reduced species are released outside the cells, where re-oxidation may occur rapidly. An alternative 'safe' bacterial pathway for reduction of metals-radionuclides has been discovered recently at the host laboratory (Dr. AC Matin). It involves proteins from the widely distributed ChrR enzyme family, performing 2 e- (4 e-) transfer processes strongly reducing oxidative stress, and leads to sequestering of reduced species. Strengthening this pathway should lessen metal toxicity and increase bioremediation capabilities. The objectives of this multidisciplinary project are (1) to assess this reduction process in bioreactors simulating waste sites conditions, using an engineered strain producing a highly improved ChrR, by analysing the oxidation state and distribution of reduced products; (2) as minor mutations of ChrR led to its increased efficiency, to test the hypothesis that improved ChrR activity is naturally present at US waste sites, where bacteria have been exposed to metals for over 50 years; (3) to test if the intra-cellular reduced products are indeed less amenable to re-oxidation than extra-cellular ones; and (4) to use the skills gained for the identification of new cytoplasmic enzymes with safe metal reduction mechanisms in uranyl-rich soils from Bessines (France), where bacterial communities with effective means of safe enzymatic reduction of uranyl are likely to have naturally evolved.'

Introduzione (Teaser)

Research into bioremediation of toxic pollutants is now making use of microbes that have evolved and survived on waste sites for more than half a century.