CHOREOGRAPHY OF HR

In vivo choreography of DNA molecules and repair proteins during the search for homology

Coordinatore	ECOLE NORMALE SUPERIEURE    more  Organization address address: "45, RUE D'ULM" city: PARIS CEDEX 05 postcode: 75230 contact info Titolo: Ms. Nome: Nathalie Cognome: Majua Email: send email Telefono: +33 1 44 32 29 00 Fax: +33 1 44 32 29 33
Nazionalità Coordinatore	France [FR]
Totale costo	242˙632 €
EC contributo	242˙632 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-IOF-2008
Funding Scheme	MC-IOF
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-01-01   -   2013-08-01

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	ECOLE NORMALE SUPERIEURE  Organization address address: "45, RUE D'ULM" city: PARIS CEDEX 05 postcode: 75230 contact info Titolo: Ms. Nome: Nathalie Cognome: Majua Email: send email Telefono: +33 1 44 32 29 00 Fax: +33 1 44 32 29 33	FR (PARIS CEDEX 05)	coordinator	242˙632.80

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Double-strand breaks (DSB) constantly challenge the integrity of the cellular genome and are important contributors to cancer and genetic diseases. Repair of damaged DNA is a dynamic process that requires careful orchestration of a multitude of enzymes and chromatin constituents that are organized into centers (foci). The outgoing laboratory recently developed powerful methods to explore the dynamics of focus assembly/disassembly in vivo. Using these techniques, I will explore one of the most intriguing steps of homologous recombination: the search for homology. During homologous recombination, the broken DNA searches for intact homologous dsDNA within the entire genome. To elucidate when and how the process of homology search occurs in vivo, I will visualise the movement of a donor and an acceptor sequence, as well as recombination factors, before, during and after double strand break repair. I will take advantage of the elegant yeast genetic system and explore the effects of targeted mutations in key enzymes involved in this process. In the return laboratory, I will to transpose the assay developed in the outgoing laboratory in mammalian cells. With the aid of the Marie Curie fellowship, I propose to spend my post-doctoral period to evolve from single molecule approaches in vitro to genetics and in vivo systems involving multiprotein complexes.'