INMARESS

In vivo magnetic resonance spectroscopy studies of muscle mitochondrial function in mice

Coordinatore	UNIVERSITA DEGLI STUDI DI MILANO    more  Organization address address: Via Festa Del Perdono 7 city: MILANO postcode: 20122 contact info Titolo: Ms. Nome: Daniela Cognome: Pozzi Email: send email Telefono: +39 02 50315383 Fax: +39 02 50315387
Nazionalità Coordinatore	Italy [IT]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2009-RG
Funding Scheme	MC-IRG
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-07-01   -   2014-06-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITA DEGLI STUDI DI MILANO  Organization address address: Via Festa Del Perdono 7 city: MILANO postcode: 20122 contact info Titolo: Ms. Nome: Daniela Cognome: Pozzi Email: send email Telefono: +39 02 50315383 Fax: +39 02 50315387	IT (MILANO)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'Alterations in muscle mitochondrial function have been implicated in the pathogenesis of numerous metabolic disorders, including insulin resistance, type 2 diabetes, obesity, and the deleterious effects of aging. However, the precise role for mitochondrial function in these processes remains to be established. In vivo 31P Magnetic Resonance Spectroscopy (31P-MRS) is an effective technique that permits the non-invasive investigation of skeletal muscle metabolism and transgenic mice are a novel tool for examining the effects of specific genes on mitochondrial function. Combining these two approaches would be a powerful methodology for studying mitochondrial function but its implementation has been limited due to the small volume of muscle from which the MR signal can be obtained and the requirement that the region of interest (ROI) must remain still for the entire duration of these lengthy studies. A new MR-compatible experimental set-up was developed to perform these experiments under low-dose anesthesia to minimize movement with constant physiological monitoring to ensure that the animal remains viable throughout the study. The unidirectional flux of ATP synthesis (Pi-->ATP) will be measured using 31P saturation-transfer MRS in differently modulated mouse models. In vivo MRS enables a unique outlook on skeletal muscle metabolism in situ. Its applications are limited not only to the study of mitochondrial function but may also be applied to other metabolic pathways.'