PHYSGENE

Dissecting a minimal genome: a physical investigation of DNA transactions in mitochondria

Coordinatore	more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	Non specificata
Totale costo	1˙497˙868 €
EC contributo	1˙497˙868 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2010-StG_20091
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-01-01   -   2015-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	VERENIGING VOOR CHRISTELIJK HOGER ONDERWIJS WETENSCHAPPELIJK ONDERZOEK EN PATIENTENZORG  Organization address address: De Boelelaan 1105 city: AMSTERDAM postcode: 1081 HV contact info Titolo: Ms. Nome: Dirkje Cognome: Schinkelshoek Email: send email Telefono: 31205987500 Fax: 31205987448	NL (AMSTERDAM)	beneficiary	0.00
2	STICHTING VU-VUMC  Organization address address: DE BOELELAAN 1105 city: AMSTERDAM postcode: 1081 HV contact info Titolo: Dr. Nome: Yvonne Cognome: Kops Email: send email Telefono: +31 20 59 87500	NL (AMSTERDAM)	hostInstitution	1˙497˙868.00
3	STICHTING VU-VUMC  Organization address address: DE BOELELAAN 1105 city: AMSTERDAM postcode: 1081 HV contact info Titolo: Prof. Nome: Gijs Jan Lodewijk Cognome: Wuite Email: send email Telefono: +31 20 5987987	NL (AMSTERDAM)	hostInstitution	1˙497˙868.00

Mappa

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 Obiettivo del progetto (Objective)

'I propose to unravel parts of the mechanochemistry of two key genome transactions: replication and transcription using an array of single-molecule techniques. To this end, I propose to study the minimal genomic machinery of mitochondria. Mitochondria are organelles in eukaryotic cells that contain their own DNA (mtDNA). Significant understanding of the biochemistry of DNA transactions in mitochondria is obtained from the in vitro reconstitution of mitochondrial replication and transcription. However, elucidating the physics of these molecular mechanisms has only just started. A major challenge of the coming decades will be dissecting and quantifying biological systems to such an extent that it makes predictive modeling possible. Single-molecule tools play a major role in this development. Hence, I plan within the scope of this proposal, to combine optical manipulation with powerful fluorescent techniques. This combination of single-molecule manipulation and fluorescence has much more potential than has been explored, so far. With the development of such tools complex biological systems can not only be controlled and measured but also visualized at the same time. The human mitochondrial genetic machinery is an ideal system for such an approach, because replication and transcription can be re-created in vitro with only seven proteins. Hence, this proposal represents a unique opportunity to quantitatively dissect a genetic machine that is actually accessible by biophysical tools. Finally, about 1 in 5000 humans suffers from a disease caused by mutations of in the mtDNA. The research proposed here will permit direct observation of proteins in action. Normal and dysfunctional proteins can thus be compared, permitting insight in the mechanistic basis of a disorder.'