NOVOSIDES

Novel Biocatalysts for the Production of Glycosides

Coordinatore	UNIVERSITEIT GENT    more  Organization address address: SINT PIETERSNIEUWSTRAAT 25 city: GENT postcode: 9000 contact info Titolo: Prof. Nome: Wim Cognome: Soetaert Email: send email Telefono: +32 9 2646083 Fax: +32 9 2646231
Nazionalità Coordinatore	Belgium [BE]
Totale costo	3˙726˙840 €
EC contributo	2˙684˙830 €
Programma	FP7-KBBE Specific Programme "Cooperation": Food, Agriculture and Biotechnology
Code Call	FP7-KBBE-2010-4
Funding Scheme	CP-FP
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-12-01   -   2014-11-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITEIT GENT  Organization address address: SINT PIETERSNIEUWSTRAAT 25 city: GENT postcode: 9000 contact info Titolo: Prof. Nome: Wim Cognome: Soetaert Email: send email Telefono: +32 9 2646083 Fax: +32 9 2646231	BE (GENT)	coordinator	787˙550.00
2	RIJKSUNIVERSITEIT GRONINGEN  Organization address address: Broerstraat 5 city: GRONINGEN postcode: 9712CP contact info Titolo: Mr. Nome: Jan Cognome: Poutsma Email: send email Telefono: +31 50 3634142 Fax: +31 50 3634500	NL (GRONINGEN)	participant	521˙000.00
3	"MIKROBIOLOGICKY USTAV - AVCR, V.V.I."  Organization address address: VIDENSKA 1083 city: PRAHA 4 postcode: 14220 contact info Titolo: Mr. Nome: Vladimir Cognome: Kren Email: send email Telefono: 420296000000 Fax: 420296000000	CZ (PRAHA 4)	participant	472˙600.00
4	FRIEDRICH-SCHILLER-UNIVERSITAT JENA  Organization address address: FURSTENGRABEN 1 city: JENA postcode: 7743 contact info Titolo: Prof. Nome: Alexander Cognome: Schiller Email: send email Telefono: +49 3641 948113 Fax: +49 3641 948102	DE (JENA)	participant	378˙880.00
5	BIO BASE EUROPE PILOT PLANT VZW  Organization address address: RODENHUIZEKAAI 1 city: GENT postcode: 9042 contact info Titolo: Ms. Nome: Sofia Cognome: Vindevogel Email: send email Telefono: +32 9 3357001 Fax: +32 9 3357002	BE (GENT)	participant	372˙000.00
6	Carbosynth Limited  Organization address address: Old Station Business Park 8 and 9 city: Compton postcode: RG206NE contact info Titolo: Dr. Nome: Vanessa Cognome: Eastwick-Field Email: send email Telefono: +44 163 557 8444 Fax: +44 163 557 9444	UK (Compton)	participant	152˙800.00

Mappa

 Word cloud

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 Obiettivo del progetto (Objective)

'This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes’ specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution.

A large and diverse collection of enzymes will first be established by screening in natural environments and by the mining of public (meta)genome databases. The enzyme collection will then be screened for activity on a variety of representative acceptors from different chemical classes. This will allow the identification of the most promising enzymes for optimisation through semi-rational and random mutagenesis. The high-throughput screening of natural and variant enzymes will be performed with newly developed fluorescent probes, that allow fast and accurate measurements of carbohydrate-active enzymes in a direct and non-destructive assay.

To achieve these ambitious goals, a complementary consortium of academia and industry has been formed that covers the whole range of required expertises. The economical potential of our technology will be demonstrated by the development and scale-up of selected glycosylation reactions at pilot-plant facilities. The produced glycosides will be actively marketed to potential end-users to promote the valorisation of the project’s results and to initiate future collaborations on novel target compounds.'

Introduzione (Teaser)

Scientists are engineering biological enzymes to catalyse an important industrial reaction known as glycosylation.