GENEPHYSCHEM

Spatio-temporal control of gene expression by physico-chemical means: from in vitro photocontrol to smart drug delivery

Coordinatore	UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	France [FR]
Totale costo	1˙450˙320 €
EC contributo	1˙450˙320 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2010-StG_20091028
Funding Scheme	ERC-SG
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-01-01   -   2015-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6  Organization address address: Place Jussieu 4 city: PARIS postcode: 75252 contact info Titolo: Dr. Nome: Damien Cognome: Baigl Email: send email Telefono: +33 1 44322431 Fax: +33 1 44322402	FR (PARIS)	hostInstitution	1˙450˙320.00
2	UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6  Organization address address: Place Jussieu 4 city: PARIS postcode: 75252 contact info Titolo: Ms. Nome: Anais Cognome: Desclos Email: send email Telefono: +33 1 44273885 Fax: +33 1 44277467	FR (PARIS)	hostInstitution	1˙450˙320.00

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 Obiettivo del progetto (Objective)

'We propose to undertake a new challenge: the control of gene expression systems by physico-chemical means to achieve the following objectives: i) developing robust tools for spatio-temporal control of protein expression; ii) understanding the role of micro-environmental factors in gene regulation; and iii) constructing and implementing in vivo smart nanomachines able to express active molecules in response to a stimulus and deliver them to a targeted cell. First, various biochemical processes (transcription, translation) will be controlled by light in vitro, based on photo-induced conformational changes of nucleic acids (DNA, RNA) and chromatin. Based on conformational changes rather than specific template-protein interaction, and combined with microfluidic methodologies, this novel approach will provide a ubiquitous tool to address gene expression using light regardless of the sequence, with unique control and spatio-temporal resolution. Second, by reconstituting photo-responsive gene expression systems in well-defined giant liposomes, we will study the dynamics of gene expression in response to light stimulation. This will allow us to establish the respective roles of the membrane (surface charge, permeability) and of the inner micro-environment composition (viscosity, molecular crowding). Third, we will develop stable, long-circulating polymer nanocapsules (polymersomes) encapsulating a gene expression material that can be triggered by light and/or molecules of biological interest. In response to the signal, an exogenous, potentially immunogenic enzyme will be expressed inside the protecting nanocapsule to locally and catalytically convert a non toxic precursor present in the medium into a cytotoxic drug that will be delivered to a cell (e.g., a cancer cell). This new concept of triggerable gene-carrying nanomachines with unique amplification capacity of drug secretion shall open new horizons for the development of smart biological probes and future therapeutics.'