LCS

Study of protein dynamics in living cells after DNA damage

Coordinatore	BIOFYZIKALNI USTAV AKADEMIE VED CESKE REPUBLIKY    more  Organization address address: Kralovopolska 135 city: Brno postcode: CZ-61265 contact info Titolo: Dr. Nome: Eva Cognome: Bartova Email: send email Telefono: +420 5 41517141 Fax: +420 5 41240498
Nazionalità Coordinatore	Czech Republic [CZ]
Totale costo	133˙000 €
EC contributo	133˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-IRSES
Funding Scheme	MC-IRSES
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-03-15   -   2014-03-14

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	BIOFYZIKALNI USTAV AKADEMIE VED CESKE REPUBLIKY  Organization address address: Kralovopolska 135 city: Brno postcode: CZ-61265 contact info Titolo: Dr. Nome: Eva Cognome: Bartova Email: send email Telefono: +420 5 41517141 Fax: +420 5 41240498	CZ (Brno)	coordinator	70˙300.00
2	UNIWERSYTET JAGIELLONSKI  Organization address address: Ul. Golebia 24 city: KRAKOW postcode: 31007 contact info Titolo: Dr. Nome: Jerzy Cognome: Dobrucki Email: send email Telefono: +48 12 6646382 Fax: +48 12 6645503	PL (KRAKOW)	participant	62˙700.00

Mappa

 Word cloud

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 Obiettivo del progetto (Objective)

'The aim of this project is to analyze dynamics of chromatin-related proteins in living cells after induced DNA damage. Cells have developed sophisticated mechanisms to overcome damage to DNA resulting in double-strand breaks (DSBs) induced by toxic agents of our environment. Improper repair of DSBs can lead to the development of cancer. Once a cancer has developed, radiation and chemotherapy are used to damage DNA in order to kill the tumor cells. Thus, the information of how cells respond to DNA damage is critical for understanding both the development of cancer and its therapy. We want to contribute to this knowledge by the elucidation of the processes changing the chromatin structure in the DSB vicinity. Chromatin changes represent the fundamental response of the cell to DSB induction that should allow the access of repair proteins to DNA for efficient signaling and repair. It should be found how the chromatin structure is modified, what is the difference in chromatin dynamics in open and compact chromatin compartments and how are all these processes coordinated in time to allow accumulation of repair proteins for full restoration of genome integrity. The synergy of Czech expertise and the experience of Polish and Russian partners in these areas is a perfect match that will allow further advancements of science. Integration of the newest equipments and technologies together: 3D Microscopy of living cells, FISH technique, immunohistochemistry, RT-PCR and Western blotting, ChIP-PCR, ChIP-on-chips, FRAP, FLIP FRET techniques (Czech and Polish partners) and Scanning Flow Cytometry, Fluorescence Microscopy (Russian partners) should give the response to reach the aim of this project.'

Introduzione (Teaser)

Understanding how cells respond to DNA damage in normal or pathological conditions is of great importance for therapy and cancer.