SHIGELLA INDUCED EMT

Diversion of adherens junctions by bacterial type III effectors during Shigella invasion of polarized intestinal epithelial cells

Coordinatore	COLLEGE DE FRANCE    more  Organization address address: Place Marcelin Berthelot 11 city: PARIS postcode: 75231 contact info Titolo: Mr. Nome: Bruno Cognome: Jeanne Email: send email Telefono: +33 1 44 27 13 13 Fax: +33 1 44 27 12 35
Nazionalità Coordinatore	France [FR]
Totale costo	100˙000 €
EC contributo	100˙000 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2010-RG
Funding Scheme	MC-IRG
Anno di inizio	2011
Periodo (anno-mese-giorno)	2011-06-01   -   2015-05-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	COLLEGE DE FRANCE  Organization address address: Place Marcelin Berthelot 11 city: PARIS postcode: 75231 contact info Titolo: Mr. Nome: Bruno Cognome: Jeanne Email: send email Telefono: +33 1 44 27 13 13 Fax: +33 1 44 27 12 35	FR (PARIS)	coordinator	100˙000.00

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 Obiettivo del progetto (Objective)

'Shigella is a gram-negative enteropathogenic bacterium responsible for dysentery in humans. The type III secretion system (T3SS) encoded on a large plasmid is a key virulence factor of Shigella. Upon epithelial contact, the T3SS allows the translocation of effector proteins into the target cells subverting host cell signal transduction pathways. These T3SS effectors reorganize the actin cytoskeleton through the targeting of RhoGTPases and tyrosine kinase signaling, leading to localized membrane ruffling and subsequent bacterial invasion of host cells. Recent evidence indicate that as opposed to what is observed in non-polarized cells, the coordinated activity of the T3SS effectors, IpgB1 and IpgB2 that activate RhoGTPases, is required for invasion of polarized intestinal epithelial cells that form highly organized intercellular junctions. Adherens junctions (AJs) are regulated by Rho GTPases and tyrosine kinases. In particular, the disruption of AJs linked to epithelial-to-mesenchymal transition (EMT) implicates signaling processes that are reminiscent to those observed during Shigella invasion. These results suggest that the diversion of AJs by these Shigella effectors is necessary for bacterial invasion. In this proposal, we will characterize signaling events mediated by T3SS effectors leading to the transient activation of an EMT-like pathway implicating RhoGTPases and tyrosine kinase signaling, accounting for the breaching of the epithelial barrier by this enteroinvasive bacterial pathogen. This proposal which addresses a key process of bacterial pathogenesis in a relevant epithelial cell system is likely to have profound implications on our understanding of mechanisms underlying the regulation of the epithelial barrier homeostasis and its manipulation by invasive pathogens.'

Introduzione (Teaser)

Shigella is a gram-negative bacterium responsible for at least 100 million cases of severe dysentery each year. Deciphering molecular mechanisms of how the bacterium invades the intestine is crucial for the development of successful strategy to fight the disease.