NANOTRAFFIC

Do small things lead to big problems? Mechanism of uptake and toxicity of metal nanoparticles in intestinal cells

 Coordinatore EIDGENOESSISCHE ANSTALT FUR WASSERVERSORGUNG ABWASSERREINIGUNG UND GEWAESSERSCHUTZ 

 Organization address address: UEBERLANDSTRASSE 133
city: DUEBENDORF
postcode: 8600

contact info
Titolo: Ms.
Nome: Bianca
Cognome: Mosimann Mottini
Email: send email
Telefono: +41 44 823 5388

 Nazionalità Coordinatore Switzerland [CH]
 Totale costo 186˙528 €
 EC contributo 186˙528 €
 Programma FP7-PEOPLE
Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call FP7-PEOPLE-2010-IEF
 Funding Scheme MC-IEF
 Anno di inizio 2012
 Periodo (anno-mese-giorno) 2012-05-01   -   2014-04-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    EIDGENOESSISCHE ANSTALT FUR WASSERVERSORGUNG ABWASSERREINIGUNG UND GEWAESSERSCHUTZ

 Organization address address: UEBERLANDSTRASSE 133
city: DUEBENDORF
postcode: 8600

contact info
Titolo: Ms.
Nome: Bianca
Cognome: Mosimann Mottini
Email: send email
Telefono: +41 44 823 5388

CH (DUEBENDORF) coordinator 186˙528.80

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

intracellular    brain    enter    tio    cu    environment    cell    human    basolateral    fish    health    apical    epithelia    spleen    ag    species    molecular    gut    mechanism    np    models    monitoring    environmental    nps    toxicity    trafficking    measured    polarized    time    liver    impact    risk    metal   

 Obiettivo del progetto (Objective)

'The nanotechnology industry offers many benefits to society, however its potential depends upon the knowledge of its impact on public health and the environment. NPs can enter the body bypassing epithelia, accumulating in spleen, gut, liver and brain as shown in fish as environmental species. While the mechanism of uptake and metabolism of NPs is unknown, their size and physical characteristics suggest that they may enter the epithelia through an endocytotic mechanism. The aim of this study is to investigate the toxic mechanism of metal- (Ag and TiO2) NPs. These NPs were selected for their impact on health and environment due to their commercially widespread use. Specific goals will be investigating NPs kinetics (uptake/trafficking/excretion) by the monitoring of NPs intracellular localization through time using polarized intestinal cells thus enabling the evaluation of apical and basolateral uptake and possible bioaccumulation effects. Toxicity of Ag- and TiO2-NPs will be evaluated by monitoring the incorporation and trafficking of vesicles containing NPs through time using state of the art electron microscopy. In parallel, biochemical and molecular cellular responses will be measured including specific effect on Cu homeostasis. Thus, Cu-ATPase function will be tested by monitoring its trafficking behaviour. In addition, Cu intracellular availability will be measured using molecular markers of Cu availability. The mechanistic knowledge generated in this project is envisioned to support human and environmental risk assessment with regard to metal ion and metal NP exposure. Moreover, the proposed development of the polarized cell line models based on the Caco2 (human) and RTgutGC (fish) cell lines for evaluating mechanism of NP interactions will not only be of great importance from a biological point of view (apical/basolateral uptake) but will also contribute to the efforts to establish animal-free, in vitro alternatives in environmental risk assessment.'

Introduzione (Teaser)

Nanoparticles (NPs) can accumulate in gut, liver, spleen and brain, as observed in fish as environmental species. An EU-funded project investigated accumulation and toxicity of silver citrate and titanium oxide NPs using fish cell culture models.

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