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N6MeA ChemSeq SIGNED

Development of chemical methods for DNA N6-methyladenine mapping

Total Cost €

EC-Contrib. €

Partnership

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N6MeA ChemSeq project word cloud

Explore the words cloud of the N6MeA ChemSeq project. It provides you a very rough idea of what is the project "N6MeA ChemSeq" about.

host protocol monomers deoxynucleosides strategies generate modifications formylcytosine biological proofs lab resolution first selectivity 5hmc methylated humans mammals outcome advantage hydroxymethyluracil labelling reductive considerably tags stalling genomic analysing strand herein pcr enriched tagging tremendous pulldown pathological efficiency reference 5hmu assisted bisulfite polymerases nucleotides of sequence selective methyladenine 5fc adapt map hydroxymethylcytosine n6mea unravel context mapping chemistry dna modification species once genome influence oxidative fragments n6 alignment sequencing chemical detection

Project "N6MeA ChemSeq" data sheet

The following table provides information about the project.

Coordinator	THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE Organization address address: TRINITY LANE THE OLD SCHOOLS city: CAMBRIDGE postcode: CB2 1TN website: www.cam.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	United Kingdom [UK]
Total cost	183˙454 €
EC max contribution	183˙454 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2017
Funding Scheme	MSCA-IF-EF-ST
Starting year	2018
Duration (year-month-day)	from 2018-04-19 to 2020-04-18

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE Organization address address: TRINITY LANE THE OLD SCHOOLS city: CAMBRIDGE postcode: CB2 1TN website: www.cam.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (CAMBRIDGE)	coordinator	183˙454.00

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Project objective

Herein, I propose to develop and adapt chemistry for the selective modification and tagging of N6-methyladenine (N6MeA) in the context of a DNA strand. Two promising chemical strategies will be applied, for which I have already established proofs-of-concept of efficiency and selectivity on DNA monomers (2'-deoxynucleosides and nucleotides). Once such a specific chemical labelling protocol has been optimised, I will use it to map N6MeA in genomic DNA with two different approaches: 1) by chemical pulldown of N6-methylated DNA fragments, sequencing of the enriched fragments, and alignment to a reference genome to generate a low-resolution N6MeA map. 2) By analysing the influence of the introduced tags and modifications on the PCR outcome and take advantage of their stalling of polymerases. The expected outcome is a first chemistry-assisted mapping of N6MeA. As for the chemical tagging of 5-formylcytosine (5fC) and 5-hydroxymethyluracil (5hmU), or oxidative and reductive bisulfite sequencing to sequence 5fC and 5-hydroxymethylcytosine (5hmC), all developed in the proposed host lab, it is expected to have a significant impact in the field. It will considerably facilitate the detection and mapping of genomic N6MeA in various species including mammals and humans, which is of tremendous importance to unravel the biological role of this DNA modification and identify novel biological as well as possible pathological pathways.

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The information about "N6MEA CHEMSEQ" are provided by the European Opendata Portal: CORDIS opendata.